The MicroRNA397b-LACCASE2 module regulates root lignification under water and phosphate deficiency
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Title |
The MicroRNA397b-LACCASE2 module regulates root lignification under water and phosphate deficiency
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Creator |
Khandal, Hitaishi
Singh, Amar Pal Chattopadhyay, Debasis |
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Subject |
Arabidopsis thaliana
miR397b lignin LACCASE2 root water-deficiency phosphate-deficiency |
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Description |
Accepted date: January 7, 2020
Deficiency of water and phosphate induce lignin deposition in roots. LACCASEs, a family of cell wall-localized multi-copper oxidases, are involved in lignin biosynthesis. We demonstrate here that LACCASE2 (LAC2) acts as a negative regulator of lignin deposition in root vascular tissues during water deficit. An Arabidopsis T-DNA insertion mutant of LAC2 displayed a short primary root and high lignin deposition in root vascular tissues. However, restoration of LAC2 expression rescued these phenotypes. LAC2 expression was significantly downregulated under water deficit and post-transcriptionally regulated by microRNA397b (miR397b) in roots under normal and water deficit conditions. Downregulation of miR397b activity increased LAC2 expression and root length, and decreased lignin content in root vasculature. Similarly, phosphate (Pi) deficiency inversely affected miR397b and LAC2 expression. Lignin deposition in the root elongation zone under Pi-limited conditions was dependent on LAC2 expression. Localized iron accumulation and callose deposition in the root elongation zone under Pi-deficiency increased with LAC2-dependent lignification, suggesting a direct relationship between these processes. Our study reveals a regulatory role for the miR397b-LAC2 module in root lignification during water- and phosphate deficiency. This work was financially supported by the Department of Biotechnology (DBT) Government of India with grants (BT/PR3304/AGR/2/815/2011 and BT/HRD/35/01/03/2014) and National Institute of Plant Genome Research, New Delhi, India (NIPGR). The project is funded by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (BT/PR3304/AGR/02/815/2011; BT/HRD/35/01/03/2014) and National Institute of Plant Genome Research, India. The assistance of central instrumentation facilities of NIPGR in various experiments is acknowledged. Authors acknowledge Dr. Sigal Savaldi Goldstein, Israel Institute of Technology-Technion, Haifa for providing facilities for some experiments. HK acknowledges Council of Scientific and Industrial Research, India (CSIR) and NIPGR for fellowship. Authors are thankful to DBT e-library Consortium (DeLCON) for providing access to e-resources. |
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Date |
2020-01-24T06:22:50Z
2020-01-24T06:22:50Z 2020 |
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Type |
Article
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Identifier |
Plant Physiology, 182(3): 1387-1403
1532-2548 DOI: https://doi.org/10.1104/pp.19.00921 http://www.plantphysiol.org/content/early/2020/01/16/pp.19.00921.long http://223.31.159.10:8080/jspui/handle/123456789/1032 |
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Language |
en_US
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Format |
application/pdf
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Publisher |
American Society of Plant Biologists
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