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The MicroRNA397b-LACCASE2 module regulates root lignification under water and phosphate deficiency

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Title The MicroRNA397b-LACCASE2 module regulates root lignification under water and phosphate deficiency
 
Creator Khandal, Hitaishi
Singh, Amar Pal
Chattopadhyay, Debasis
 
Subject Arabidopsis thaliana
miR397b
lignin
LACCASE2
root
water-deficiency
phosphate-deficiency
 
Description Accepted date: January 7, 2020
Deficiency of water and phosphate induce lignin deposition in roots. LACCASEs, a family of cell wall-localized multi-copper oxidases, are involved in lignin biosynthesis. We demonstrate here that LACCASE2 (LAC2) acts as a negative regulator of lignin deposition in root vascular tissues during water deficit. An Arabidopsis T-DNA insertion mutant of LAC2 displayed a short primary root and high lignin deposition in root vascular tissues. However, restoration of LAC2 expression rescued these phenotypes. LAC2 expression was significantly downregulated under water deficit and post-transcriptionally regulated by microRNA397b (miR397b) in roots under normal and water deficit conditions. Downregulation of miR397b activity increased LAC2 expression and root length, and decreased lignin content in root vasculature. Similarly, phosphate (Pi) deficiency inversely affected miR397b and LAC2 expression. Lignin deposition in the root elongation zone under Pi-limited conditions was dependent on LAC2 expression. Localized iron accumulation and callose deposition in the root elongation zone under Pi-deficiency increased with LAC2-dependent lignification, suggesting a direct relationship between these processes. Our study reveals a regulatory role for the miR397b-LAC2 module in root lignification during water- and phosphate deficiency.
This work was financially supported by the Department of Biotechnology (DBT)
Government of India with grants (BT/PR3304/AGR/2/815/2011 and BT/HRD/35/01/03/2014)
and National Institute of Plant Genome Research, New Delhi, India (NIPGR). The project is funded by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (BT/PR3304/AGR/02/815/2011; BT/HRD/35/01/03/2014)
and National Institute of Plant Genome Research, India. The assistance of central
instrumentation facilities of NIPGR in various experiments is acknowledged. Authors
acknowledge Dr. Sigal Savaldi Goldstein, Israel Institute of Technology-Technion, Haifa for
providing facilities for some experiments. HK acknowledges Council of Scientific and
Industrial Research, India (CSIR) and NIPGR for fellowship. Authors are thankful to DBT e-library Consortium (DeLCON) for providing access to e-resources.
 
Date 2020-01-24T06:22:50Z
2020-01-24T06:22:50Z
2020
 
Type Article
 
Identifier Plant Physiology, 182(3): 1387-1403
1532-2548
DOI: https://doi.org/10.1104/pp.19.00921
http://www.plantphysiol.org/content/early/2020/01/16/pp.19.00921.long
http://223.31.159.10:8080/jspui/handle/123456789/1032
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists