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Title Shelf life extension of tuna fillets by gelatin and chitosan based edible coating incorporated with clove oil
 
Names Kumar, K.S.
Chrisolite, B.
Sugumar, G.
Bindu, J.
Venkateshwarlu, G.
Date Issued 2018 (iso8601)
Abstract The present study aimed to develop bioactive edible
coating (BEC) solutions from gelatin and chitosan,
incorporated with different concentrations of clove
oil as a natural preservative and evaluate their effect
on shelf life of tuna fillets. The antibacterial activity
against 11 fish spoilage and fish-borne bacteria were
tested by agar well diffusion method, minimum
inhibitory concentration (MIC) and minimum bactericidal
concentration (MBC). Gram positive bacteria
were more susceptible than gram negative
bacteria. Among all the tested bacteria, Bacillus
cereus and Staphylococcus aureus were most sensitive
to BEC solutions. The tuna fillets were dipped in
BEC solutions with different concentrations viz., 1%
Acetic acid, 1% Chitosan (C), 1% Gelatin + 1% Clove
oil (GC), 1% Chitosan + 1% Clove oil (CC), 1%
Gelatin + 1% Chitosan + 1% Clove oil (GCC) and
changes in APC, TBARS, TVB-N and sensory values
were studied during storage under refrigerated
condition (4°C). The fillets without dip treatment
was considered as control. Dip treatment of BEC
solutions significantly delayed the rate of microbial
spoilage and extended the shelf life of tuna fillets
by six days during refrigerated storage. Solutions
incorporated with clove oil, especially fillets treated
with 1% gelatin + 1% chitosan + 1% clove oil (GCC)
solution had better in-vitro antimicrobial properties
and showed excellent preservative action on tuna
fillets. The BEC solutions incorporated with clove
oil demonstrated its potential as an excellent natural
antibacterial agent which can be used as an effective
alternative to synthetic antibacterial agents and could be used for packaging of tuna and other
fishery products.
Genre Article
Identifier Fishery Technology 55(2):104-113