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Root-specific expression of chickpea cytokinin oxidase/dehydrogenase 6 leads to enhanced root growth, drought tolerance and yield without compromising nodulation

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Title Root-specific expression of chickpea cytokinin oxidase/dehydrogenase 6 leads to enhanced root growth, drought tolerance and yield without compromising nodulation
 
Creator Khandal, Hitaishi
Gupta, Santosh Kumar
Dwivedi, Vikas
Mandal, Drishti
Sharma, Nilesh Kumar
Vishwakarma, Niraj Kumar
Pal, Lalita
Choudhary, Megha
Francis, Aleena
Malakar, Paheli
Singh, Nagendra Pratap
Sharma, Kapil
Sinharoy, Senjuti
Singh, Narendra Pratap
Sharma, Rameshwar
Chattopadhyay, Debasis
 
Subject CaCKX6
Cicer arietinum L
cytokinin
drought-tolerance
mineral
root
 
Description Accepted date: 17 March 2020
Cytokinin group of phytohormones regulate root elongation and branching during post‐embryonic development. Cytokinin degrading enzymes cytokinin oxidases/dehydrogenases (CKXs) have been deployed to investigate biological activities of cytokinin and to engineer root growth. We expressed chickpea cytokinin oxidase 6 (CaCKX6) under the control of a chickpea root‐specific promoter of CaWRKY31 in Arabidopsis thaliana and chickpea having determinate and indeterminate growth patterns, respectively, to study the effect of cytokinin depletion on root growth and drought tolerance. Root‐specific expression of CaCKX6 led to a significant increase in lateral root number and root biomass in Arabidopsis and chickpea without any penalty to vegetative and reproductive growth of shoot. Transgenic chickpea lines showed increased CKX activity in root. Soil‐grown advanced chickpea transgenic lines exhibited higher root‐to‐shoot biomass ratio and enhanced long‐term drought tolerance. These chickpea lines were not compromised in root nodulation and nitrogen fixation. The seed yield in some lines was up to 25% higher with no penalty in protein content. Transgenic chickpea seeds possessed higher levels of zinc, iron, potassium and copper. Our results demonstrated the potential of cytokinin level manipulation in increasing lateral root number and root biomass for agronomic trait improvement in an edible legume crop with indeterminate growth habit.
The project is funded by the Department of Biotechnology (DBT), Ministry of Science and
Technology, Government of India (BT/PR3304/AGR/02/815/2011 and BT/HRD/35/01/03/2014 to
DC) and (Grant no. BT/PR11671/PBD/16/828/2008 to RS) and National Institute of Plant Genome Research, India. LP, MC & PM is supported by SRF & JRF fellowship from Council of
Scientific and Industrial Research (CSIR, Govt. of India). The funders had no role in study design,
data collection and analysis, decision to publish, or preparation of the manuscript. The assistance
of central instrumentation facilities of NIPGR in various experiments is acknowledged. The
authors are grateful to the DBT-eLibrary Consortium (DeLCON) for providing access to eResources. The authors have no conflicts of interest to declare.
 
Date 2020-03-30T15:40:25Z
2020-03-30T15:40:25Z
2020
 
Type Article
 
Identifier Plant Biotechnology Journal, 18(11): 2225-2240
1467-7652
https://doi.org/10.1111/pbi.13378
https://onlinelibrary.wiley.com/doi/10.1111/pbi.13378
http://223.31.159.10:8080/jspui/handle/123456789/1049
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons