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Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]

OAR@ICRISAT

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Relation http://oar.icrisat.org/11467/
https://doi.org/10.1016/j.jcs.2019.102850
doi:10.1016/j.jcs.2019.102850
 
Title Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]
 
Creator Kotla, A
Phuke, R
Hariprasanna, K
Mehtre, S P
Rathore, A
Gorthy, S
Srivastava, R K
Das, R
Bhanu Prakash, A
Radhika, K
Hash, C T
Reddy, B V S
Patil, J V
Jabeen, F
Shashikanth, D
Jaganathan, J
Gaddameedi, A
Subhasini, V
Deshpande, S
Kumar, A A
 
Subject Sorghum
Genetics and Genomics
Food and Nutrition
 
Description Sorghum is a major food crop in the semi-arid tropics of Africa and Asia. Enhancing the grain iron (Fe) and zinc (Zn) concentration in sorghum using genetic approaches would help alleviate micronutrient malnutrition in millions of poor people consuming sorghum as a staple food. To localize genomic regions associated with grain Fe and Zn, a sorghum F6 recombinant inbred line (RIL) population (342 lines derived from cross 296B PVK 801) was phenotyped in six environments, and genotyped with simple sequence repeat (SSR), DArT (Diversity Array Technology) and DArTSeq (Diversity Array Technology) markers. Highly significant genotype environment interactions were observed for both micronutrients. Grain Fe showed greater variation than Zn. A sorghum genetic map was constructed with 2088 markers (1148 DArTs, 927 DArTSeqs and 13 SSRs) covering 1355.52 cM with an average marker interval of 0.6 cM. Eleven QTLs (individual) and 3 QTLs (across) environments for Fe and Zn were identified. We identified putative candidate genes from the QTL interval of qfe7.1, qzn7.1, and qzn7.2 (across environments) located on SBI-07 involved in Fe and Zn metabolism. These were CYP71B34, and ZFP 8 (ZINC FINGER PROTEIN 8). After validation, the linked markers identified in this study can help in developing high grain Fe and Zn sorghum cultivars in sorghum improvement programs globally.
 
Publisher Elsevier
 
Date 2019-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11467/1/2019_IdentificationofQTLsandcandidategenesforhighgrainFeandZnconcentrationinsorghumSorghumbicolorL.Moench.pdf
Kotla, A and Phuke, R and Hariprasanna, K and Mehtre, S P and Rathore, A and Gorthy, S and Srivastava, R K and Das, R and Bhanu Prakash, A and Radhika, K and Hash, C T and Reddy, B V S and Patil, J V and Jabeen, F and Shashikanth, D and Jaganathan, J and Gaddameedi, A and Subhasini, V and Deshpande, S and Kumar, A A (2019) Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]. Journal of Cereal Science (TSI), 90. pp. 1-9. ISSN 0733-5210