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Title Valorization of fish viscera for crude proteases production and its use in bioactive protein hydrolysate preparation
 
Names Murthy, L.N.
Phadke, G.G.
Parvathy, U.
Jeyakumari, A.
Joshy, C.G.
Zynudheen, A.A.
Ravishankar, C.N.
Date Issued 2017 (iso8601)
Abstract Increasing environmental pollution and limited
biological resources has emphasized the need for better
utilization of fish processing waste. In the present study,
visceral proteases from little tuna (Euthynnus affinis),
catla (Catla catla) and tilapia (Oreochromis mossambicus)
belonging to different habitats using acetone, ethanol and
ammonium sulfate fractional precipitation were isolated
and characterized. Enzymes precipitated from little tuna
and tilapia exhibited higher specific activity at 40% saturation
for ammonium sulfate fractional precipitation and
the specific activities were 18.19 and 13.67 U/mg for little
tuna and tilapia enzymes, respectively. Enzymes precipitated
from catla reported higher specific activity of 8.32 U/
mg at 60% saturation for ammonium sulfate fractional precipitation.
Acetone precipitated fraction exhibited higher
recovery for all crude enzymes studied. The effect of different
combinations of crude tuna visceral waste enzyme
and papain enzyme on hydrolysate properties was optimized
using response surface methodology. Second order
response surface regression model was used to explain the
total variability in terms of quadratic and interaction effects
of combination enzyme concentration on the properties
of croaker protein hydrolysate with a significant R2
value
for protein content (0.92), tyrosine content (0.99), degree
of hydrolysis (0.99), DPPH free radical scavenging activity
(0.97), ferric reducing antioxidant power (0.93), metal chelating activity (0.88), foaming capacity (0.92) and foam
stability (0.88). Based on the desirability score, papain at
0.81% concentration and crude tuna visceral protease at
4.36% was found to be optimal for achieving desired properties
of croaker protein hydrolysate.
Genre Article
Identifier Waste & Biomass Valorization 4:1-12