Record Details

A Prospective study to evaluate the demographic variation of gender independent sequences in cell-free fetal DNA (cffDNA) concentration and to predict pregnancy outcomes by non-kit based economical method

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement A Prospective study to evaluate the demographic variation of gender independent sequences in cell-free fetal DNA (cffDNA) concentration and to predict pregnancy outcomes by non-kit based economical method
 
Added Entry - Uncontrolled Name Mriganka Mouli, Saha
Subir Kumar, Das
Madhumita, Mukhyopadhyay
Maitree, Bhattacharrya
 
Uncontrolled Index Term Cell-free fetal DNA (cffDNA); Hypermethylated DNA; Preeclampsia; Trophoblast
 
Summary, etc. This gender-independent detection of cell-free fetal DNA in maternal plasma using RASSF1A/β-actin has curtained off a new dimension regarding its utility to predict the adverse pregnancy outcomes. Recent efforts have been directed at developing sequences from cell-free fetal DNA (cffDNA) as markers for pregnancy outcomes. The utility of cffDNA using the methylation-dependent DSCR3 and RASSF1A markers along with total cell-free DNA (cf-DNA) in maternal serum by HYP2 marker are useful in predicting adverse pregnancy outcomes. Increased amount (>95th percentile) of cffDNA fraction in the second trimester is associated with preterm birth. Indigenously developed low-cost method of the gender-independent sequence markers from cffDNA was investigated and evaluated with the standardized commercial kits as predictive markers for adverse pregnancy outcomes. Our results indicated that indigenously developed method for detection of geneder-independent cffDNA can be applicable for screening test of adverse pregnancy outcome.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-05-12 19:14:14
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/35182
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); ##issue.vol## 57, ##issue.no## 2 (2020): IJBB Vol.57(2) [April 2020]
 
Language Note en