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Genome-wide microsatellite markers in castor (Ricinus communis L.): Identification, development, characterization, and transferability in Euphorbiaceae

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Title Genome-wide microsatellite markers in castor (Ricinus communis L.): Identification, development, characterization, and transferability in Euphorbiaceae
 
Creator Dharajiya, Darshan T.
Shah, Anshuman
Galvadiya, Bhemji P.
Patel, M.P.
Srivastava, Rishi
Pagi, Nalin K.
Solanki, S.D.
Parida, Swarup K.
Tiwari, Kapil K.
 
Subject Castor (Ricinus communis L.)
Euphorbiaceae
Genome-wide microsatellites
Cross transferability
 
Description Accepted date: 7 April 2020
Castor (Ricinus communis L.; 2n = 20) belongs to family Euphorbiaceae, is an industrially important non-edible
oilseed crop which is widely cultivated in arid and semi-arid regions of the world. The demand for castor has
kept increasing in the international market and to meet the demand, enhancement of genetic improvement in
castor is very essential. Genome-wide availability of molecular markers is prerequisite for rapid genetic improvement of castor. Therefore, genome-wide microsatellite motifs from the draft genome of castor were
identified (37,972) and utilized for primer designing in the present study. Among identified microsatellites, direpeats (25,144) were most abundant followed by tri-repeats (10,636). A set of 304 primers was used for the
validation of microsatellites markers in four genotypes of castor. Total 198 (65.13%) primers were amplified,
among them 103 (52.02%) primers were polymorphic. Moreover, a set of 29 polymorphic microsatellites primer
pairs was used for the cross-genera transferability study in five species of Euphorbiaceae viz., Jatropha multifida
L., Euphorbia antiquorum L., E. milii Des Moul., E. thymifolia L., and E. neriifolia L. The maximum amplification of
68.97% was observed in E. antiquorum and minimum amplification of 31.03% was reported in J. multifida.
Identified primers will facilitate to access the genetic diversity analysis, DNA fingerprinting, variety/hybrid
identification, linkage map constructions, marker assisted breeding (MAB), association mapping, the discovery
of quantitative trait loci (QTLs), genes among castor genotypes, and various species of Euphorbiaceae.
The authors are thankful to the authorities of CPCA, SDAU,
Sardarkrushinagar, Gujarat, India, for providing facilities to conduct
the research. The authors are also thankful to the Government of
Gujarat, Indiafor providing the financial support.
 
Date 2020-05-12T14:13:42Z
2020-05-12T14:13:42Z
2020
 
Type Article
 
Identifier Industrial Crops and Products, 151: 112461
0926-6690
https://doi.org/10.1016/j.indcrop.2020.112461
https://www.sciencedirect.com/science/article/pii/S0926669020303770
http://223.31.159.10:8080/jspui/handle/123456789/1061
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.