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| Field | Value |
| Title | Functional characterization of Trehalose biosynthesis genes from E.coil: An osmolyte involved in stress tolerance |
| Names |
Joseph, T.C.
Rajan, L.A. Thampuran, N. James, R. |
| Date Issued | 2010 (iso8601) |
| Abstract | Trehalose (1-a-D-glucopyranosyl-1-a-D-glucopyranoside), a non-reducing disaccharide is a major compatible solute, which maintains fluidity of membranes and protects the biological structure of organisms under stress. In this study, trehalose-6-phosphate synthase (otsA) and trehalose-6-phosphate phosphatase (otsB) genes encoding for trehalose biosynthesis from Escherichia coli was cloned as an operon and expressed in E. coli M15(pREP4). The recombinant E. coli strain showed a threefold increase in the activity of otsBA pathway enzymes, compared to the control strain. The transgenic E. coli accumulated up to 0.86 mg/l of trehalose. The sequence of otsA and ots B genes reported in this study contains several base substitutions with that of reported sequences in GenBank,resulting in the altered amino acid sequences of the translated proteins. |
| Genre | Article |
| Topic | E. coli |
| Identifier | Molecular Biotechnology 2010: 46(1), 20-25 |