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Title Nitrification potential of Achromobacter xylosoxidans isolated from fresh water finfish farms of Kerala, India
 
Names Basha, K.A.
Joseph, T.C.
Lalitha, K.V.
Vineetha, D.
Rathore, G.
Tripati, G.
Prasad, K.P.
Date Issued 2018 (iso8601)
Abstract A heterotrophic strain ACM-1, isolated from Pangasius spp fish farms showed an ability
to convert ammonium to nitrate under aerobic conditions. Based on the molecular
phylogenetic analysis 16S rRNA gene sequence ACM-1 matched similarity to
Achromobacter xylosoxidans (100%). ACM-1 showed amplification for Betaproteobacterial
16S AOB gene (465 bp) confirming the characteristic feature of ammonia
oxidation. Sodium pyruvate (1%) was confirmed as the probable carbon source for the
luxurious growth of Achromobacter xylosoxidans among the six carbon sources tested. At
30oC, 5% preculture and 120 rpm in shaker, ACM-1 could able to remove 100%
ammonium within 36h. The ammonium removal rate was reported to be 1.3 NH4
+ -N mg
L-1h-1 at 30oC. The rate of ammonium removal at different temperature (20oC, 25oC, 37oC)
ranged between 0.9 to1.33 NH4
+-N mg L-1 h-1. The nitrite produced during this experiment
remained at constant rate and nitrate concentration increased after the initial hours of
experiment. Therefore, the results demonstrate that Achromobacter xylosoxidans ACM-1
can remove ammonium in batch cultures and could be applied in aquaculture farms as a
biological nitrifier.
Genre Article
Identifier Int. J. Curr. Microbiol. Appl. Sci. 7(8):2645-2654