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Title Isolation and characterisation of vibrio parahaemolyticus from seafoods along the southwest coast of India
 
Names Chakraborty, D.R.
Surendran, P.K.
Joseph, T.C.
Date Issued 2008 (iso8601)
Abstract The work was aimed to study the microbial
quality of the seafood sold in the domestic markets and incidence
of Vibrio parahaemolyticus. Samples comprising of
shellfish, finfish, and cephalopodswere collected fromvarious
fish markets in and around Cochin. Presumed V. parahaemolyticus
were identified by standard biochemical tests, and
further confirmed by polymerase chain reaction targeting
species-specific tl gene (450 bp). About 81% of the samples
were found to exceed the limits specified for total plate count
while total presumptiveV. parahaemolyticus countwas above
the limit in 71% of the samples ranging from 5.5 9 105 to
9.7 9 107 and 0.31 9 102 to 7.8 9 106 cfu/g, respectively.
Pathogenicity of the identified isolates was confirmed by
Kanagawa phenomenon and urease activity.Atotal of 10%of
the isolates exhibited weak haemolysis on Wagatsuma agar,
and 1% of the isolates showed urease activity using Christensen’s
urea agar. Random amplified polymorphic DNA
analysis revealed two major clusters based on the species
rather than seasonality. The gel pattern revealed 8–10 bands
ranging from 0.45 to 3.0 kb. Antibiogram results revealed
85% of the strains sensitive to chloramphenicol and nitrofurantoin.
Multiple antibiotic resistance index was found to be 0.4 thus suggesting the risk potential involved in consuming seafoods. The present study has clearly demonstrated the need to adopt seafood safety measures for the products meant for human consumption.
Genre Article
Topic Vibrio parahaemolyticus
Identifier World Journal of Microbiology and Biotechnology 2008: 24, 2045-2054