Record Details


Field	Value

Title	Assessment of the pathogenicity of cell culture-adapted Newcastle disease virus strain Komarov

Names	Visnuvinayagam, S. Thangavel, K. Lalitha, N. Malmarugan, S. Sukumar, K.
Date Issued	2015 (iso8601)
Abstract	Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Eggadapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-weekold chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18th (final) passage. Thus, a tissue- culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.
Genre	Article
Topic	Newcastle disease
Identifier	Brazilian Journal of Microbiology 2015:46(3), 861-865

ICAR Research Data Repository for Knowledge Management