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A potent enzybiotic against methicillin-resistant Staphylococcus aureus.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A potent enzybiotic against methicillin-resistant Staphylococcus aureus.
 
Creator Kaur, Jasjeet
Singh, Prashant
Sharma, Deepak
Harjai, Kusum
Chhibber, Sanjay
 
Subject QR Microbiology
 
Description Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls for the development of alternative and effective modalities against MDR S. aureus. Endolysins are bacteriophage-derived antibacterials, which attack essential conserved elements of peptidoglycan that are vital for bacterial survival, making them promising alternatives or complements to existing antibiotics for tackling such infections. For developing endolysin lysin-methicillin-resistant-5 (LysMR-5) as an effective antimicrobial agent, we evaluated its physical and chemical characteristics, and its intrinsic antibacterial activity against staphylococcal strains, including methicillin-resistant Staphylococcus aureus (MRSA). In this study, we cloned, expressed, and purified LysMR-5 from S. aureus phage MR-5. In silico analysis revealed that LysMR-5 harbors two catalytic and one cell wall-binding domain. Biochemical characterization and LC-MS analysis showed that both catalytic domains were active and had no dependence on divalent ions for their action, Zn exerted a negative effect. The optimal lytic activity of the endolysin was at 37 °C/pH 7.0 and in the presence of ≥ 300 mM concentration of NaCl. Circular dichroism (CD) demonstrated a loss in secondary structure with an increase in temperature confirming the thermosensitive nature of endolysin. Antibacterial assays revealed that LysMR-5 was active against diverse clinical isolates of staphylococci. It showed high lytic efficacy against S. aureus ATCC 43300, as an endolysin concentration as low as 15 µg/ml was sufficient to achieve maximum lytic activity within 30 min and it was further confirmed by scanning electron microscopy. Our results indicate that rapid and strong bactericidal activity of LysMR-5 makes it a valuable candidate for eradicating multidrug-resistant S. aureus.
 
Publisher Springer
 
Date 2020-08-04
 
Type Article
PeerReviewed
 
Relation https://doi.org/10.1007/s11262-020-01762-4
http://crdd.osdd.net/open/2570/
 
Identifier Kaur, Jasjeet and Singh, Prashant and Sharma, Deepak and Harjai, Kusum and Chhibber, Sanjay (2020) A potent enzybiotic against methicillin-resistant Staphylococcus aureus. Virus genes, 56 (4). pp. 480-497. ISSN 1572-994X