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Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses

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Title Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses
 
Creator Tinega, A.N.
Pelle, Roger
Kang'a, S.
Gicheru, M.M.
Taracha, E.L.N.
Nene, Vishvanath
Graham, S.P.
 
Subject THEILERIA PARVA
PROTEINS
PEPTIDES
CELLS
 
Description Alex N. Tinega, Roger Pellé, Evans L.N. Taracha & Simon P. Graham are ILRI authors.
Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-γ ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo.
 
Date 2009-11-02T04:36:13Z
2009-11-02T04:36:13Z
2009-07-15
 
Type Journal Article
 
Identifier Tinega,, A.N.; Pellé, R..; Kang’a, S.; Gicheru,, M.M.; Taracha, E.L.N.; Nene, V.; Graham, S.P. 2009. Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses. Veterinary Immunology and Immunopathology (The Netherlands). v. 130(1-2). p. 107-113.
0165-2427
http://hdl.handle.net/10568/50
 
Language en
 
Source Veterinary Immunology and Immunopathology