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Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva

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Title Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva
 
Creator Hemmink, Johanneke D.
Weir, W.
MacHugh, Niall D.
Graham, S.P.
Patel, Ekta
Paxton, E.
Shiels, B.
Toye, Philip G.
Morrison, W.I.
Pelle, Roger
 
Subject animal diseases
vaccines
research
infectious diseases
parasitology
 
Description An infection and treatment protocol is used to vaccinate cattle against Theileria parva infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions provides high levels of protection, some animals are not protected against challenge with buffalo-derived T. parva. Knowledge of the genetic composition of the Muguga cocktail vaccine is required to understand how vaccination is able to protect against field challenge and to identify the potential limitations of the vaccine. The aim of the current study was to determine the extent of genetic and antigenic diversity within the parasite isolates that constitute the Muguga cocktail. High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and mini-satellite loci. The former focused on genes encoding CD8+ T cell antigens, believed to be relevant to protective immunity. The results demonstrate that each of the three component stocks of the cocktail contains limited parasite genotypic diversity, with single alleles detected at many gene/satellite loci and, moreover, that two of the components show a very high level of similarity. Thus, the vaccine incorporates very little of the genetic and antigenic diversity observed in field populations of T. parva. The presence of alleles at low frequency (
 
Date 2016-07
2016-05-05T05:43:08Z
2016-05-05T05:43:08Z
 
Type Journal Article
 
Identifier Hemmink, J.D., Weir, W., MacHugh, N.D., Graham, S.P., Patel, E., Paxton, E., Shiels, B., Toye, P.G., Morrison, W.I. and Pelle, R. 2016. Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva. International Journal for Parasitology 46(8):495–506.
0020-7519
https://hdl.handle.net/10568/73303
https://doi.org/10.1016/j.ijpara.2016.02.007
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 495-506
 
Publisher Elsevier BV
 
Source International Journal for Parasitology