Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya
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Title |
Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya
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Creator |
Ndungu, S.M.
Messmer, M.M. Ziegle, D. Thuita, Moses N. Vanlauwe, Bernard Frossard, Emmanuel Thonar, C. |
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Subject |
bradyrhizobium
cowpeas nodulation proteins bacteroid biotechnology |
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Description |
Cowpea N2 fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a field trial in Mbeere during the 2014 short rains. Strains from bacteroids of cowpea nodules from pot and field experiments were accurately identified as Bradyrhizobium by MALDI-TOF based on the SARAMIS™ database. In the field, abundant indigenous populations 7.10 × 103 rhizobia g−1 soil, outcompeted introduced strains. As revealed by MALDI-TOF, indigenous strains clustered into six distinct groups (I, II, III, IV, V and VI), group III were most abundant occupying 80% of nodules analyzed. MALDI-TOF was rapid, affordable and reliable to identify Bradyrhizobium strains directly from nodule suspensions in competition pot assays and in the field with abundant indigenous strains thus, its suitability for future competition assays. Evaluating strain competitiveness and then symbiotic efficacy is proposed in bioprospecting for potential cowpea inoculant strains.
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Date |
2018-06
2018-05-23T12:43:07Z 2018-05-23T12:43:07Z |
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Type |
Journal Article
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Identifier |
Ndungu, S.M., Messmer, M.M., Ziegler, D., Thuita, M., Vanlauwe, B., Frossard, E. & Thonar, C. (2018). Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya. Applied Microbiology and Biotechnology, 1-14.
0175-7598 https://hdl.handle.net/10568/92850 https://doi.org/10.1007/s00253-018-9005-6 NATURAL RESOURCE MANAGEMENT |
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Language |
en
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Rights |
Copyrighted; all rights reserved
Limited Access |
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Format |
5265-5278
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Publisher |
Springer Science and Business Media LLC
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Source |
Applied Microbiology and Biotechnology
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