ICAR Research Data Repository for Knowledge Management
Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
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View Archive Info| Field | Value | |
| Title |
Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
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| Creator |
Pinel-Galzi, A.
Hébrard, E. Traoré, O. Silué, D. Albar, L. |
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| Subject |
research
rice |
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| Description |
Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and characterization of resistance sources. This paper describes a protocol for mechanical inoculation of rice seedlings with RYMV and two methods of resistance evaluation – one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA).
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| Date |
2018
2019-07-02T14:57:02Z 2019-07-02T14:57:02Z |
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| Type |
Journal Article
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| Identifier |
Pinel-Galzi, A., Hébrard, E., Traoré, O., Silué, D., and Albar, L. 2018. Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings. Bio-protocol 8(11):e2863
https://hdl.handle.net/10568/102052 https://doi.org/10.21769/BioProtoc.2863 |
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| Language |
en
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| Rights |
Copyrighted; all rights reserved
Limited Access |
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| Publisher |
Bio-Protocol, LLC
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| Source |
Bio-Protocol
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