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Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

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Title Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.
 
Creator Kreuze, J.F.
Pérez, A.
Untiveros, M.
Quispe, D.
Fuentes, S.
Barker, I.
Simon, R.
 
Subject GENOMES
VIROSES
DIAGNOSIS
PLANT VIRUSES
SWEET POTATOES
 
Description We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known viral pathogens, occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge.
Peer Review
 
Date 2017-02-07T13:03:33Z
2017-02-07T13:03:33Z
2009-05-25
 
Type Journal Article
 
Identifier Kreuze, J.F.; Perez, A.; Untiveros, M.; Quispe, D.; Fuentes, S.; Barker, I.; Simon, R. 2009. Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses. Virology. (USA). ISSN 0042-6822. 388(1):1-7.
0042-6822
https://hdl.handle.net/10568/79776
 
Language en
 
Format 1-7
 
Source Virology