Evolution of quality standards in plant cryobanks - The International Potato Center as a case study
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Title |
Evolution of quality standards in plant cryobanks - The International Potato Center as a case study
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Creator |
Vollmer, R.
Ellis, David Barkley, N.A. |
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Subject |
potatoes
cryopreservation organization of research gene banks |
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Description |
The International Potato Center holds one of the largest and most diverse potato cryobanks worldwide. The cryo-collection includes 1855 landraces, belonging to seven potato species and four different ploidy levels. The potato cryo-collection was principally built up in recent years (2013-17), under strict quality and operative standards. The average regrowth rate of the potato cryo-collection is 58% and 1234 of 1855 accessions showed a regrowth rate of 50% or higher. The success rate, i.e. the percent of successfully cryopreserved accessions (minimum 20% of regrowth) in relation to the totally processed accessions has increased in 2016 to 95.4%. Real time data record of each step of the process with pocket PCs and bi-dimensional cryo-resistant barcode labels is an important part of our cryo-system. Full plant recovery (+LN) as standard parameter for regrowth assessment, a large-scale applicable cryo-protocol (high success rate), systematic viability reassessment, clear threshold of minimum acceptable regrowth (30%: one single cryo-run, 20-30%: two independent cryo-runs,
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Date |
2018-02
2018-04-03T19:10:51Z 2018-04-03T19:10:51Z |
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Type |
Journal Article
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Identifier |
Vollmer, R.; Ellis, D.; Barkley, N. 2018. Evolution of quality standards in plant cryobanks - The International Potato Center as a case study. Cryobiology. (USA). ISSN 0011-2240. 80:163.
0011-2240 https://hdl.handle.net/10568/92010 https://doi.org/10.1016/j.cryobiol.2017.10.036 |
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Language |
en
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Rights |
Copyrighted; all rights reserved
Open Access |
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Format |
163
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Publisher |
Elsevier BV
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Source |
Cryobiology
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