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Evolution of quality standards in plant cryobanks - The International Potato Center as a case study

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Title Evolution of quality standards in plant cryobanks - The International Potato Center as a case study
 
Creator Vollmer, R.
Ellis, David
Barkley, N.A.
 
Subject potatoes
cryopreservation
organization of research
gene banks
 
Description The International Potato Center holds one of the largest and most diverse potato cryobanks worldwide. The cryo-collection includes 1855 landraces, belonging to seven potato species and four different ploidy levels. The potato cryo-collection was principally built up in recent years (2013-17), under strict quality and operative standards. The average regrowth rate of the potato cryo-collection is 58% and 1234 of 1855 accessions showed a regrowth rate of 50% or higher. The success rate, i.e. the percent of successfully cryopreserved accessions (minimum 20% of regrowth) in relation to the totally processed accessions has increased in 2016 to 95.4%. Real time data record of each step of the process with pocket PCs and bi-dimensional cryo-resistant barcode labels is an important part of our cryo-system. Full plant recovery (+LN) as standard parameter for regrowth assessment, a large-scale applicable cryo-protocol (high success rate), systematic viability reassessment, clear threshold of minimum acceptable regrowth (30%: one single cryo-run, 20-30%: two independent cryo-runs,
 
Date 2018-02
2018-04-03T19:10:51Z
2018-04-03T19:10:51Z
 
Type Journal Article
 
Identifier Vollmer, R.; Ellis, D.; Barkley, N. 2018. Evolution of quality standards in plant cryobanks - The International Potato Center as a case study. Cryobiology. (USA). ISSN 0011-2240. 80:163.
0011-2240
https://hdl.handle.net/10568/92010
https://doi.org/10.1016/j.cryobiol.2017.10.036
 
Language en
 
Rights Copyrighted; all rights reserved
Open Access
 
Format 163
 
Publisher Elsevier BV
 
Source Cryobiology