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Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia

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Title Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia
 
Creator Ortiz, Diana Carolina
Bohórquez Cháux, Adriana
Duque E., Myriam Cristina
Tohme, Joseph M.
Cuéllar, David
Mosquera Vasquez, Teresa
 
Subject aflp
passiflora edulis
genetic variation
plantations
variacion genética
plantaciones
 
Description This study evaluated P. edulis f. edulis intraspecific variation at DNA level using AFLPs and SSRs. About 60 specimens were collected from Colombian commercial plantations in the departments of Antioquia, Boyacá, Cundinamarca, Huila, Quindío and Tolima. Ten specimens were also included from Corpoica’s La Selva research center Passiflora germplasm bank. A non-commercial purple passion fruit plant and three species from the Passiflora genus (Passiflora maliformis, Passiflora edulis f. flavicarpa and Passiflora ligularis) were included as controls. The six most informative ones were selected from the initial screening of 49 different AFLP primer combinations; 52–92 DNA fragments for each combination in each genotype (total 419 fragments) were obtained by using these six combinations. 17 SSR primers reported in P. edulis f. flavicarpa and Passiflora alata were tested on purple passion fruit. Eight of them were successfully amplified but none showed polymorphism. Relationships among individuals were assessed on AFLP data using cluster analysis by Dice coefficient, UPGMA algorithm and the multiple correspondence analysis ordination method based on Greenacre measurement. The similarity coefficients ranged from 0.75 to 1.00 giving an average of 0.96 within the different purple passion fruit materials, showing low genetic variability for the purple passion fruit material used in this study.
 
Date 2012-08
2014-09-24T08:42:06Z
2014-09-24T08:42:06Z
 
Type Journal Article
 
Identifier Ortiz, Diana Carolina; Bohórquez Cháux, Adriana; Duque E., Myriam Cristina; Tohme, Joseph M.; Cuéllar, David; Mosquera Vásquez, Teresa. 2012. Evaluating purple passion fruit (Passiflora edulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia . Genetic Resources and Crop Evolution (Netherlands) 59:1089-1099.
1573-5109
https://hdl.handle.net/10568/43422
https://doi.org/10.1007/s10722-011-9745-y
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Publisher Springer Science and Business Media LLC
 
Source Genetic Resources and Crop Evolution