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A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.

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Title A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
 
Creator Njukeng, A.P.
Ariri, G.I.
Hughes, Jacqueline d'Arros
Agindoran, B.O.
Mignouna, H.D.
Thottappilly, G.
 
Subject YAMS
PLANT VIRUSES
MONOCLONAL ANTIBODIES
DIOSCOREA
 
Description A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by fusion of FOX-NY myeloma cells with immune splenocytes from in vivo immunizations of a Balb/C mouse. The polyclonal antiserum to YMV and the Mab (YMV-M24) were used in developing a triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) system for the detection of YMV from samples collected from fields. The test gave a positive reaction even at a sap dilution of 1:6250-1:31250 from leaf samples collected from different plants species, while antigen-coated plant (ACP) ELISA using the Mab could detect only to a dilution of 1:1250. TAS-ELISA was more sensitive and reliable when compared with ACP-ELISA using YMV-M24. YMV-M24 detected YMV isolates in some Dioscorea species from seven countries in the main yam growing area of West Africa.
Peer Review
 
Date 2019-09-12T08:32:00Z
2019-09-12T08:32:00Z
2002
 
Type Journal Article
 
Identifier Njukeng, A.P., Atiri, G.I., D'A Hughes, J., Agindotan, B.O., Mignouna, H.D. & Thottappilly, G. (2002). A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. Tropical Science, 42(2), 65-74.
0041-3291
https://hdl.handle.net/10568/103631
 
Language en
 
Rights Copyrighted; all rights reserved
 
Format 65-74
 
Source Tropical Science