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R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1

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Title R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1
 
Creator Roman, M.L.
Izarra, M.
Lindqvist-Kreuze, Hanele
Rivera, C.
Gamboa, S.
Tovar, J.C.
Forbes, G.A.
Kreuze, J.F.
Ghislain, M.
 
Subject POTATOES
PHYTOPHTHORA INFESTANS
DISEASE RESISTANCE
TRANSGENICS
 
Description The Avr avirulence gene of Phytophthora infestans and R gene of the potato are the genetic components of the gene-for-gene interaction resulting in host plant resistance. This effector-triggered immunity has been recently exploited to generate extreme resistance to late blight in potato by genetic engineering. The choice of the R genes, their number forming a R gene stack, and the pathogen Avr gene diversity will likely determine how long this extreme resistance will last. Here, we report on a comparative study on the Rpi-vnt1.1 gene which originated from Solanum venturii, and was introduced in the potato variety ‘Desiree’ by genetic transformation, and the Avr-vnt1 gene from two isolates of the EC-1 lineage of P. infestans. EC-1 was reported previously as not expressing the Avr-vnt1 gene and, therefore, being virulent on Rpi-vnt1.1 transgenic plants. Unexpectedly, whole-plant resistance assays identified 5 out of 52 transgenic events as resistant to two isolates of P. infestans, POX067 and POX109, belonging to the EC-1 lineage. We demonstrated that in both isolates, the Avr-vnt1.1 gene was expressed at a low level. Expression of the Rpi-vnt1.1 gene was shown to be rapidly increased by two-fold and subsequently to have steady state expression for at least 5 days after the inoculation. The Rpi-vnt1.1 gene in addition to other R genes as a stack in farmers’ preferred varieties will confer extreme resistance to late blight disease and rotations of plants with different R-gene-stack in time is likely to last longer than plants with single R gene.
United States Agency for International Development
2Blades Foundation
Peer Review
 
Date 2018-05-28T19:20:01Z
2018-05-28T19:20:01Z
2017
 
Type Journal Article
 
Identifier Roman, M.L.; Izarra, M.; Lindqvist-Kreuze, H.; Rivera, C.; Gamboa, S.; Tovar, J.C.; Forbes, G.A.; Kreuze, J.; Ghislain, M. 2017. R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1. Plant Cell, Tissue and Organ Culture. (Netherlands). ISSN 0167-6857. 131(2):259-268.
0167-6857
https://hdl.handle.net/10568/92940
 
Language en
 
Format 259-268
 
Source Plant Cell, Tissue and Organ Culture