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Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing

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Title Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing
 
Creator Silva, G.
Bömer, M.
Rathnayake, A.I.
Sewe, S.O.
Visendi, P.
Oyekanmi, J.O.
Quain, M.D.
Akomeah, B.
Kumar, P. Lava
Seal, S.E.
 
Subject rna
betaflexiviridae
yams
viruses
nigeria
ghana
genomes
molecular markers
 
Description To date, several viruses of different genera have been reported to infect yam (Dioscorea spp.). The full diversity of viruses infecting yam, however, remains to be explored. High-throughput sequencing (HTS) methods are increasingly being used in the discovery of new plant viral genomes. In this study, we employed HTS on yam to determine whether any undiscovered viruses were present that would restrict the international distribution of yam germplasm. We discovered a new virus sequence present in 31 yam samples tested and have tentatively named this virus “yam virus Y” (YVY). Twenty-three of the samples in which YVY was detected showed mosaic and chlorotic leaf symptoms, but Yam mosaic virus was also detected in these samples. Complete genome sequences of two YVY viral isolates were assembled and found to contain five open reading frames (ORFs). ORF1 encodes a large replication-associated protein, ORF2, ORF3 and ORF4 constitute the putative triple gene block proteins, and ORF5 encodes a putative coat protein. Considering the species demarcation criteria of the family Betaflexiviridae, YVY should be considered as a novel virus species in the family Betaflexiviridae. Further work is needed to understand the association of this new virus with any symptoms and yield loss and its implication on virus-free seed yam production.
 
Date 2019-06
2019-06-25T12:45:36Z
2019-06-25T12:45:36Z
 
Type Journal Article
 
Identifier Silva, G., Bömer, M., Rathnayake, A.I., Sewe, S.O., Visendi, P., Oyekanmi, J. O., ... & Seal, S.E. (2019). Molecular characterization of a new virus species identified in yam (Dioscorea spp.) by high-throughput sequencing. Plants, 8(6), 167-176.
2223-7747
https://hdl.handle.net/10568/101684
https://doi.org/10.3390/plants8060167
PLANT PRODUCTION & HEALTH
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 167-176
application/pdf
 
Publisher MDPI AG
 
Source Plants