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Comparison of rice lines derived through anther culture and the pedigree method in relation to blast (Pyricularia grisea Sacc.) resistance

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Title Comparison of rice lines derived through anther culture and the pedigree method in relation to blast (Pyricularia grisea Sacc.) resistance
 
Creator Martínez Racines, César Pompilio
Correa Victoria, Fernando J.
Amézquita, María Cristina
Tulande Valencia, Edgar
Lema, G
Zeigler, Robert S.
 
Subject ORYZA SATIVA
RICE
EVALUATION
DISEASE RESISTANCE
PYRICULARIA GRISEA
ANTHER CULTURE
ORYZA SATIVA
ARROZ
EVALUACIÓN
RESISTENCIA A LA ENFERMEDAD
PYRICULARIA GRISEA
CULTIVO DE ANTERAS
 
Description CIAT- Outstanding Research Publication Award (ORPA) - 1996
Crosses were made between Fanny (highly susceptible to blast) and 11 cultivars differing in blast resistance. Using the pedigree method (PM) segregating generations were evaluated and selected for blast resistance. Via anther culture (AC), doubled-haploids were obtained from F1 plants and from F2 blast-susceptible plants. Pedigree and anther culture-derived lines were planted together and evaluated for blast resistance under rainfed conditions at the Santa Rosa Experiment Station, Villavicencio, Colombia. The principal objective was to compare PM and AC in terms of their efficiency in producing rice lines resistant to blast. Results of a stratified analysis showed an association between method and blast resistance. Results of the logit-model analysis showed that AC produced a significantly (P=0.0001) higher proportion of lines with initial blast resistance (leaf- and neck-blast reaction ?4) than did PM across all cross types. Stable blast resistance was assessed based on field performance over 3 years. AC was superior to PM in generating stable resistance for only some cross types. Consequently, with a few exceptions, AC can be used as effectively as PM to develop rice cultivars resistant to blast, with savings in time and labor. Additionally, blast-resistant lines were obtained either by the pedigree method or by anther culture from crosses between blast-susceptible cultivars (Fanny/CICA4 and Fanny/Colombial). This excludes somaclonal variation as a possible mechanism responsible for this resistance and suggests that a recombination of minor genes could have occurred and was fixed through either method. However, the stability of the resistance was greater in pedigree-derived lines. The implications of these findings for rice blast-resistance breeding are discussed.
Peer-reviewed
 
Date 2014-09-24T08:41:54Z
2014-09-24T08:41:54Z
1996
 
Type Journal Article
 
Identifier Martínez, César; Correa V. , Fernando; Amézquita, María C.; Tulande, Edgar; Lema, German; Zeigler, Robert (1996). Comparison of Rice Lines Derived through Anher Culture and the Pedigree Method in Relation to Blast (Pyricularia grisea Sacc.) Resistance. Theoretical and Applied Genetics, 92: 583-590
1432-2242
https://hdl.handle.net/10568/43288
 
Language en
 
Source Theoretical and Applied Genetics