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Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America

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Title Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America
 
Creator Morales, Francisco José
Ward, Elaine
Castaño, Mauricio
Arroyave, José A.
Lozano Potes, Iván
Adams, Michael J.
 
Subject ORYZA SATIVA
POLYMYXA
FUROVIRUSES
PLASMODIOPHOROMYCETS
PLANT DISEASES
ORYZA SATIVA
POLYMYXA
FUROVIRUS
PLASMODIOPHOROMYCETES
ENFERMEDADES DE LAS PLANTAS
 
Description CIAT- Outstanding Research Publication Award (ORPA) - 2000
A new disease of rice, known as ‘entorchamiento’ (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts and purified preparations from diseased rice plants, contained virus-like particles ca. 20?nm in diameter, with a bimodal length of 260 and 360?nm. Particle aggregates were also observed in the cytoplasm of infected rice leaf cells. Electrophoretic analyses of purified preparations and dsRNA extracts, revealed a single protein species of M 22,500, and four dsRNA bands ca. 6300, 4600, 2700 and 1800?bp in size. Cystosori, characteristic of plasmodiophorid fungal vectors of plant viruses, were consistently observed in the roots of diseased rice plants. PCR and sequence analyses of amplified fungal DNA products from infected rice roots, revealed that the putative fungus vector was Polymyxa graminis. A Western blot of tissue extracts obtained from ‘crinkled’ rice plants from Colombia, using antiserum against a West African isolate of rice stripe necrosis furovirus (RSNV), resulted in the detection of a protein band of approximately M 22,000. The RSNV antiserum recognized the Colombian virus isolate in serologically specific electron microscopy tests. These results confirm the presence of RSNV in the Americas.
Peer-reviewed
 
Date 2014-09-24T08:42:05Z
2014-09-24T08:42:05Z
2000
 
Type Journal Article
 
Identifier Morales, Francisco José; Ward, Elaine; Castaño, Mauricio; Arroyave, José A.; Lozano Potes, Iván; Adams, Michael J. (2000). Emergence and Partial Characterization of Rice Stripe Necrosis Virus and its Fungus Vector in South America. European Journal of Plant Pathology 105: 643-650
1573-8469
https://hdl.handle.net/10568/43405
 
Language en
 
Source European Journal of Plant Pathology