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In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches

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Title In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches
 
Creator Lorieux, Mathias
Blein, Mélisande
Lozano, Jaime
Bouniol, Matthieu
Droc, Gaëtan
Diévart, Anne
Périn, Christophe
Mieulet, Delphine
Lanau, Nadege
Bès, Martine
Rouvière, Claire
Gay, Céline
Piffanelli, Pietro
Larmande, Pierre
Michel, Corinne
Barnola, Isabelle
Biderre-Petit, Corinne
Sallaud, Christophe
Pérez, Pascual
Bourgis, Fabienne
Ghesquière, Alain
Gantet, Pascual
Tohme, Joseph M.
Morel, Jean-Benoit
Guiderdoni, Emmanuel
 
Subject oryza sativa
rice
mutagenesis
molecular genetics
phenotypes
dna
arroz
mutagénesis
genética molecular
fenotipos
adn
 
Description CIAT- Outstanding Research Publication Award (ORPA) - 2012
We report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals—i.e. one‐fourth to one‐fifth of the estimated rice nontransposable element gene complement—are interrupted by sequence‐indexed T‐DNA (6563 genes) and/or Tos17 (2755 genes) inserts. Six hundred and forty‐three genes are interrupted by both T‐DNA and Tos17 inserts. High quality of the sequence indexation of the T2 seed samples was ascertained by several approaches. Field evaluation under agronomic conditions of 27 832 OTL has revealed that 18.2% exhibit at least one morphophysiological alteration in the T1 progeny plants. Screening 10 000 lines for altered response to inoculation by the fungal pathogen Magnaporthe oryzae allowed to observe 71 lines (0.7%) developing spontaneous lesions simulating disease mutants and 43 lines (0.4%) exhibiting an enhanced disease resistance or susceptibility. We show here that at least 3.5% (four of 114) of these alterations are tagged by the mutagens. The presence of allelic series of sequence‐indexed mutations in a gene among OTL that exhibit a convergent phenotype clearly increases the chance of establishing a linkage between alterations and inserts. This convergence approach is illustrated by the identification of the rice ortholog of AtPHO2, the disruption of which causes a lesion‐mimic phenotype owing to an over‐accumulation of phosphate, in nine lines bearing allelic insertions.
 
Date 2012-06
2017-10-12T08:02:05Z
2017-10-12T08:02:05Z
 
Type Journal Article
 
Identifier Lorieux, Mathias; Blein, Mélisande; Lozano, Jaime; Bouniol, Matthieu; Droc, Gaëtan; Diévart, Anne; Périn, Christophe; Mieulet, Delphine; Lanau, Nadege; Bès, Martine; Rouvière, Claire; Gay, Céline; Piffanelli, Pietro; Larmande, Pierre; Michel, Corinne; Barnola, Isabelle; Biderre-Petit, Corinne; Sallaud, Christophe; Perez, Pascual; Bourgis, Fabienne; Ghesquière, Alain; Gantet, Pascual; Tohme, Joseph M.; Morel, Jean Benoit; Guiderdoni, Emmanuel. 2012. In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches. Plant Biotechnology Journal. 10(5) 555-568.
https://hdl.handle.net/10568/88270
https://doi.org/10.1111/J.1467-7652.2012.00689.x
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Publisher Wiley
 
Source Plant Biotechnology Journal