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Fruit set and plant regeneration in cassava following interspecific pollination with castor bean

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Title Fruit set and plant regeneration in cassava following interspecific pollination with castor bean
 
Creator Baguma, J.K.
Mukasa, S.B.
Kawuki, R.S.
Tugume, A.K.
Buttibwa, M.
Nalela, P.
Eyokia, M.
Oshaba, B.
Ceballos, Hernán
Lentini, Z.
Baguma, Y.
 
Subject cassava
manihot esculenta
plant breeding
varieties
ploidy
ploidía
 
Description The increasing demand for cassava (Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid genetic improvement. This study aimed at inter-pollinating cassava with castor bean (Ricinus communis), with a purpose of inducing and regenerating cassava doubled haploids (DHs). A total of 3,349 flowers from twelve elite cassava varieties were inter-pollinated with caster bean. A total of 803 fruits were harvested for early embryo rescue and/or ovule culture. Of these, three were dissected to obtain seven unique embryos, while 800 were dissected to obtain 1312 young ovules, all of which were cultured in vitro. Overall, 82 (6.25%) of the cultured ovules formed callus that originated from the embryosac region, which is haploid. Four out of seven rescued embryos (57.1%) regenerated into plantlets. Ploidy analyses of 24 samples using flow cytometry revealed that 23 of the analysed samples were diploid. However, one callus sample was anueploid. Only one sample had an exceptionally high level of homozygosity ( 84.2%). These findings lay a foundation for future research aimed at induction of haploids in cassava.
 
Date 2019
2019-10-24T16:42:23Z
2019-10-24T16:42:23Z
 
Type Journal Article
 
Identifier Baguma, J.K.; Mukasa, S.B. ; Kawuki, R.; Tugume, A.K. ; Buttibwa, M. ; Nalela, P. ; Eyokia, M. ; Oshaba, B. ; Ceballos, Hernan ; Lentini, Z. & Baguma, Y. (2019). Fruit set and plant regeneration in cassava following interspecific pollination with castor bean. African Crop Science Journal. 27(1): 99-118.
1021-9730
https://hdl.handle.net/10568/105509
https://doi.org/10.4314/acsj.v27i1.8
 
Language en
 
Rights CC-BY-NC-4.0
Open Access
 
Format 99-118
 
Publisher African Journals Online (AJOL)
 
Source African Crop Science Journal