Record Details

Artificial microRNA-derived resistance to Cassava brown streak disease

CGSpace

View Archive Info
 
 
Field Value
 
Title Artificial microRNA-derived resistance to Cassava brown streak disease
 
Creator Wagaba, Henry
Patil, Basavaprabhu L.
Mukasa, Settumba
Alicai, Titus
Fauquet, Claude M.
Taylor, Nigel J.
 
Subject CASSAVA BROWN STREAK DISEASE
CASSAVA BROWN STREAK VIRUS
MANIHOT ESCULENTA
PLANT VIRUSES
RNA SEQUENCE
VIRUSES
GENOMES
DISEASE CONTROL
RESISTANCE
VIRUS DE LAS PLANTAS
SECUENCIA DE ARN
VIRUS
GENOMAS
CASSAVA
CONTROL DE ENFERMEDADES
RESISTENCIA
 
Description Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3′-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20–60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava.
Peer Review
 
Date 2016-03-28T20:23:26Z
2016-03-28T20:23:26Z
2016-02-18
 
Type Journal Article
 
Identifier Wagaba, Henry; Patil, Basavaprabhu L.; Mukasa, Settumba; Alicai, Titus; Fauquet, Claude M.; Taylor, Nigel J.. 2016. Artificial microRNA-derived resistance to Cassava brown streak disease. Journal of Virological Methods 231: 38-43.
0166-0934
https://hdl.handle.net/10568/72739
 
Language en
 
Format 231: 38-43
 
Source Journal of Virological Methods