Record Details

Development and field performance of nitrogen use efficient rice lines for Africa

CGSpace

View Archive Info
 
 
Field Value
 
Title Development and field performance of nitrogen use efficient rice lines for Africa
 
Creator Selvaraj, Michael Gomez
Valencia, Milton Orlando
Ogawa, Satoshi
Lu, Yingzhi
Wu, Liying
Downs, Christopher
Skinner, Wayne
Lu, Zhongjin
Kridl, Jean C.
Ishitani, Manabu
Boxtel, Jos van
 
Subject oryza sativa
rice
alanine aminotransferase
field experimentation
grain
yield
transgenics
nitrogen fertilizers
transformation
arroz
alanina aminotransferasa
experimentación en campo
granos
rendimiento
transgénicos
abonos nitrogenados
 
Description Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Africa 4) rice lines overexpressing barley alanine amino transferase (HvAlaAT) under the control of a rice stress-inducible promoter (pOsAnt1). Field evaluations over three growing seasons and two rice growing ecologies (lowland and upland) revealed that grain yield of pOsAnt1:HvAlaAT transgenic events was significantly higher than sibling nulls and wild-type controls under different N application rates.
Our field results clearly demonstrated that this genetic modification can significantly increase the dry biomass and grain yield compared to controls under limited N supply. Increased yield in transgenic events was correlated with increased tiller and panicle number in the field, and evidence of early establishment of a vigorous root system in hydroponic growth. Our results suggest that expression of the HvAlaAT gene can improve NUE in rice without causing undesirable growth phenotypes. The NUE technology described in this article has the potential to significantly reduce the need for N fertilizer and simultaneously improve food security, augment farm economics and mitigate greenhouse gas emissions from the rice ecosystem.
 
Date 2017-06
2017-01-30T19:05:54Z
2017-01-30T19:05:54Z
 
Type Journal Article
 
Identifier Gomez Selvaraj, Michael; Valencia, Milton Orlando; Ogawa, Satoshi; Lu, Yingzhi; Wu, Liying; Downs, Christopher; Skinner, Wayne; Lu, Zhongjin; Kridl, Jean C.; Ishitani, Manabu; Boxtel, Jos Van. 2017. Development and field performance of nitrogen use efficient rice lines for Africa . Plant Biotechnology Journal . Wiley-Blackwell Publishing Ltd., 15(6): 775-787
1467-7644
https://hdl.handle.net/10568/79451
https://doi.org/10.1111/pbi.12675
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 15(6): 775-787
 
Publisher Wiley
 
Source Plant Biotechnology Journal