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Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field

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Title Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field
 
Creator Selvaraj, Michael Gomez
Ishizaki, Takuma
Valencia, Milton Orlando
Ogawa, Satoshi
Dedicova, Beata
Ogata, Takuya
Yoshiwara, Kyouko
Maruyama, Kyonoshin
Kusano, Miyako
Saito, Kazuki
Takahashi, Fuminori
Shinozaki, Kazuo
Nakashima, Kazuo
Ishitani, Manabu
 
Subject arabidopsis thaliana
oryza sativa
rice
drought stress
drought tolerance
genotypes
transgenics
yield
arroz
estrés de sequia
transgénicos
rendimiento
plant breeding
fitomejoramiento
 
Description Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed Ubi:AtGolS2 transgenic lines also had higher levels of galactinol than the non-transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress.
 
Date 2017-11
2017-04-07T19:47:26Z
2017-04-07T19:47:26Z
 
Type Journal Article
 
Identifier Gomez Selvaraj, Michael; Ishizaki, Takuma; Valencia, Milton; Ogawa, Satoshi; Dedicova, Beata; Ogata, Takuya; Yoshiwara, Kyouko; Maruyama, Kyonoshin; Kusano, Miyako; Saito, Kazuki; Takahashi, Fuminori; Shinozaki, Kazuo; Nakashima, Kazuo; Ishitani, Manabu. 2017. Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field . Plant Biotechnology Journal .15(11): 1465-1477.
1467-7644
https://hdl.handle.net/10568/80683
https://doi.org/10.1111/pbi.12731
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 15(11): 1465-1477
 
Publisher Wiley
 
Source Plant Biotechnology Journal