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Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage

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Title Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage
 
Creator Ghneim-Herrera, Thaura
Selvaraj, Michael Gomez
Meynard, Donaldo
Fabre, Denis
Peña, Alexandra
Romdhane, Walid Ben
Saad, Rania Ben
Ogawa, Satoshi
Rebolledo, María Camila
Ishitani, Manabu
Tohme, Joseph M.
Al-Doss, Abdullah
Guiderdoni, Emmanuel
Hassairi, Afif
 
Subject oryza sativa
rice
drought stress
drought tolerance
transgénicos
transgenics
yield
arroz
estrés de sequia
rendimiento
 
Description We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imposed at the reproductive stage. In all trials, the two transgenic lines, RN5 and RN6, consistently out-performed non-transgenic (NT) and wild-type (WT) controls, providing 50–90% increases in grain yield (GY). Enhancement of tillering and panicle fertility contributed to this improved GY under drought. In contrast with physiological records collected during previous greenhouse dry-down experiments, where drought was imposed at the early tillering stage, we did not observe significant differences in photosynthetic parameters, leaf water potential, or accumulation of antioxidants in flag leaves of AlSAP-lines subjected to drought at flowering. However, AlSAP expression alleviated leaf rolling and leaf drying induced by drought, resulting in increased accumulation of green biomass. Therefore, the observed enhanced performance of the AlSAP-lines subjected to drought at the reproductive stage can be tentatively ascribed to a primed status of the transgenic plants, resulting from a higher accumulation of biomass during vegetative growth, allowing reserve remobilization and maintenance of productive tillering and grain filling. Under irrigated conditions, the overall performance of AlSAP-lines was comparable with, or even significantly better than, the NT and WT controls. Thus, AlSAP expression inflicted no penalty on rice yields under optimal growth conditions. Our results support the use of AlSAP transgenics to reduce rice GY losses under drought conditions.
 
Date 2017
2017-06-12T21:23:13Z
2017-06-12T21:23:13Z
 
Type Journal Article
 
Identifier Ghneim-Herrera, Thaura; Gomez Selvaraj, Michael; Meynard, Donaldo; Fabre, Denis; Peña, Alexandra; Romdhane, Walid Ben; Saad, Rania Ben; Ogawa, Satoshi; Rebolledo, Maria C.; Ishitani, Manabu; Tohme, Joe; Al-Doss, Abdullah; Guiderdoni, Emmanuel; Hassairi, Afif. 2017. Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage . Frontiers in Plant Science . 8: 994. https://doi.org/10.3389/fpls.2017.00994
1664-462X
https://hdl.handle.net/10568/81532
https://doi.org/10.3389/fpls.2017.00994
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 8: 994
 
Publisher Frontiers Media SA
 
Source Frontiers in Plant Science