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Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS)

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Title Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS)
 
Creator Ikeogu, Ugochukwu N.
Davrieux, Fabrice
Dufour, D.L.
Ceballos, H.
Egesi, Chiedozie N.
Jannink, Jean-Luc
 
Subject cassava
manihot esculenta
carotenoids
infrared spectrophotometry
specific gravity
high performance liquid chromatography
cloning
phenotypes
carotenoides
espectroscopia infrarroja
peso específico
cromatografía líquida de alta resolución
cromatografía líquida alta presión
clonación
 
Description Portable Vis/NIRS are flexible tools for fast and unbiased analyses of constituents with minimal sample preparation. This study developed calibration models for dry matter content (DMC) and carotenoids in fresh cassava roots using a portable Vis/NIRS system. We examined the effects of eight data pre-treatment combinations on calibration models and assessed calibrations on processed and intact root samples. We compared Vis/NIRS derived-DMC to other phenotyping methods. The results of the study showed that the combination of standard normal variate and de-trend (SNVD) with first derivative calculated on two data points and no smoothing (SNVD+1111) was adequate for a robust model. Calibration performance was higher with processed than the intact root samples for all the traits although intact root models for some traits especially total carotenoid content (TCC) (R2c = 96%, R2cv = 90%, RPD = 3.6 and SECV = 0.63) were sufficient for screening purposes. Using three key quality traits as templates, we developed models with processed fresh root samples. Robust calibrations were established for DMC (R2c = 99%, R2cv = 95%, RPD = 4.5 and SECV = 0.9), TCC (R2c = 99%, R2cv = 91%, RPD = 3.5 and SECV = 2.1) and all Trans β-carotene (ATBC) (R2c = 98%, R2cv = 91%, RPD = 3.5 and SECV = 1.6). Coefficient of determination on independent validation set (R2p) for these traits were also satisfactory for ATBC (91%), TCC (88%) and DMC (80%). Compared to other methods, Vis/NIRS-derived DMC from both intact and processed roots had very high correlation (>0.95) with the ideal oven-drying than from specific gravity method (0.49). There was equally a high correlation (0.94) between the intact and processed Vis/NIRS DMC. Therefore, the portable Vis/NIRS could be employed for the rapid analyses of DMC and quantification of carotenoids in cassava for nutritional and breeding purposes.
 
Date 2017-12-11
2018-01-04T19:00:02Z
2018-01-04T19:00:02Z
 
Type Journal Article
 
Identifier Ikeogu, Ugochukwu N.; Davrieux, Fabrice; Dufour, Dominique; Ceballos, Hernan; Egesi, Chiedozie N.; Jannink, Jean-Luc. 2017. Rapid analyses of dry matter content and carotenoids in fresh cassava roots using a portable visible and near infrared spectrometer (Vis/NIRS) . PLoS ONE 12(12): e0188918.
https://hdl.handle.net/10568/89923
https://doi.org/10.1371/journal.pone.0188918
 
Language en
 
Rights CC0-1.0
Open Access
 
Format e0188918
 
Publisher Public Library of Science (PLoS)
 
Source PLOS ONE