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An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)

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Title An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)
 
Creator Behnam, Babak
Bohórquez Cháux, Adriana
Castañeda Méndez, Oscar F.
Tsuji, Hiroyuki
Ishitani, Manabu
Becerra López Lavelle, Luis Augusto
 
Subject manihot esculenta
rna
cassava
plant breeding
yield
 
Description We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170–600 μg/g) using low‐cost chemicals and consumables and with minimal contamination from polysaccharides, polyphenols, proteins, and other secondary metabolites. In particular, A260:A280 ratios were >2.0 for RNA from various tissues, and all of the present RNA samples yielded ribosomal integrity number values of greater than six. The resulting high purity and quality of isolated RNAs will facilitate downstream applications (quantitative reverse transcriptase‐polymerase chain reaction or RNA sequencing) in cassava molecular breeding.
 
Date 2019-04
2018-11-28T19:07:24Z
2018-11-28T19:07:24Z
 
Type Journal Article
 
Identifier Behnam, Babak; Bohorquez-Chaux, Adriana; Castañeda-mendez, Oscar Fernando; Tsuji, Hiroyuki; Ishitani, Manabu & Becerra Lopez-Lavalle, Luis Augusto (2018). An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz), FEBS Open Bio. In press
2211-5463
https://hdl.handle.net/10568/98372
https://doi.org/10.1002/2211-5463.12561
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format In press
 
Publisher Wiley
 
Source FEBS Open Bio