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Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

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Title Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle
 
Creator Kaba, S.A.
Musoke, A.J.
Schaap, D.
Schetters, T.
Rowlands, G.J.
Vermeulen, A.N.
Nene, Vishvanath
Vlak, J.M.
Oers, M.M. van
 
Subject CATTLE
EAST COAST FEVER
BACULOVIRIDAE [INVERTEBRATE VIRUSES]
THEILERIA PARVA
VACCINES
ELISA
ANTIBODIES
SPOROZOA
 
Description Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67DeltaSS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100mug per dose, which is a major improvement over previous recombinant p67 products.
 
Date 2013-07-03T05:26:14Z
2013-07-03T05:26:14Z
2005
 
Type Journal Article
 
Identifier Vaccine;23(21): 2791-2800
0264-410X
https://hdl.handle.net/10568/33217
 
Language en
 
Source Vaccine