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A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing

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Title A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing
1-38
 
Creator Untiveros, M.
Olspert, A.
Firth, A.E.
Artola, K.
Kreuze, Jan F.
Valkonen, J.P.T.
 
Subject sweet potatoes
plant viruses
gene expression
molecular biology
 
Description The single‐stranded, positive‐sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3′ third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the ‘transframe’ product, P1N‐PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N‐PISPO inhibited short‐distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co‐opted for the evolution and expression of further novel gene products.
 
Date 2016-09
2016-02-01T19:51:32Z
2016-02-01T19:51:32Z
 
Type Journal Article
 
Identifier Untiveros, M.; Olspert, A.; Artola, K.; Firth, A.E.; Kreuze, J.F.; Valkonen, J.P.T. 2016. A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing. Molecular Plant Pathology. (UK). ISSN 1464-6722. 17(7):1111-1123.
1364-3703
https://hdl.handle.net/10568/70230
https://doi.org/10.1111/mpp.12366
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 1111-1123
 
Publisher Wiley
 
Source Molecular Plant Pathology