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A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat

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Title A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat
 
Creator Kidane, Yosef Gebrehawaryat
Gesesse, C.A.
Hailemariam, B.N.
Desta, E.A.
Mengistu, D.K.
Fadda, Carlo
Pe, M.E.
Dell'Acqua, M.
 
Subject quantitative trait loci
plant breeding
smallholders
tetraploidy
wheat
genomes
 
Description The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with an international elite durum wheat variety (Asassa). The Ethiopian NAM population (EtNAM) is composed of fifty interconnected bi-parental families, totalling 6280 recombinant inbred lines (RILs) that represent both a powerful quantitative trait loci (QTL) mapping tool, and a large pre-breeding panel. Here, we discuss the molecular and phenotypic diversity of the EtNAM founder lines, then we use an array featuring 13 000 single nucleotide polymorphisms (SNPs) to characterize a subset of 1200 EtNAM RILs from 12 families. Finally, we test the usefulness of the population by mapping phenology traits and plant height using a genome wide association (GWA) approach. EtNAM RILs showed high allelic variation and a genetic makeup combining genetic diversity from Ethiopian FVs with the international durum wheat allele pool. EtNAM SNP data were projected on the fully sequenced AB genome of wild emmer wheat, and were used to estimate pairwise linkage disequilibrium (LD) measures that reported an LD decay distance of 7.4 Mb on average, and balanced founder contributions across EtNAM families. GWA analyses identified 11 genomic loci individually affecting up to 3 days in flowering time and more than 1.6 cm in height. We argue that the EtNAM is a powerful tool to support the production of new durum wheat varieties targeting local and global agriculture.
 
Date 2019-07
2019-11-26T10:16:36Z
2019-11-26T10:16:36Z
 
Type Journal Article
 
Identifier Kidane, Y.G.; Gesesse, C.A.; Hailemariam, B.N.; Desta, E.A.; Mengistu, D.K.; Fadda, C.; Pe, M.E.; Dell'Acqua, M. (2019). A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat. Plant Biotechnology Journal 17(7) p. 1380–1393. ISSN: 1467-7644
1467-7644
https://hdl.handle.net/10568/105890
https://doi.org/10.1111/pbi.13062
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 1380-1393
application/pdf
 
Publisher Wiley
 
Source Plant Biotechnology Journal