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Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

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Title Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
 
Creator Thekisoe, O.M.M.
Rambritch, N.E.
Nakao, R.
Bazie, R.S.
Mbati, P.
Namangala, B.
Malele, I.
Skilton, Robert A.
Jongejan, F.
Sugimoto, C.
Kawazu, S.I.
Inoue, N.
 
Subject theileria parva
east coast fever
 
Description We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries.
 
Date 2010-01
2009-12-30T09:00:14Z
2009-12-30T09:00:14Z
 
Type Journal Article
 
Identifier Thekisoe, O.M.M.; Rambritch, N.E.; Nakao, R.; Bazie, R.S.; Mbati, P.; Namangala, B.; Malele, I.; Skilton, R.A.; Jongejan, F.; Sugimoto, C.; Kawazu, S.I.; Inoue, N. 2009. Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes. International Journal for Parasitology. v. 40(1). p. 55-61.
0020-7519
https://hdl.handle.net/10568/308
https://doi.org/10.1016/j.ijpara.2009.07.004
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 55-61
 
Publisher Elsevier
 
Source International Journal for Parasitology