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The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number

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Title The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number
 
Creator Collins, N.E.
Liebenberg, J.
Villiers, Etienne P. de
Brayton, K.A.
Louw, E.
Pretorius, E.
Faber, F.E.
Heerden, H. van
Josemans, A.
Kleef, M. van
Steyn, H.C.
Strijp, M.F. van
Zweygarth, E.
Jongejan, F.
Maillard, J.C.
Berthier, D.
Botha, M.
Joubert, F.
Corton, C.H.
Thomson, N.R.
Allsopp, M.T.
Allsopp, B.A.
 
Subject ehrlichia ruminantium
animal health
animal diseases
 
Description Heartwater, a tick-borne disease of domestic and wild ruminants, is caused by the intracellular rickettsia Ehrlichia ruminantium (previously known as Cowdria ruminantium). It is a major constraint to livestock production throughout subSaharan Africa, and it threatens to invade the Americas, yet there is no immediate prospect of an effective vaccine. A shotgun genome sequencing project was undertaken in the expectation that access to the complete protein coding repertoire of the organism will facilitate the search for vaccine candidate genes. We report here the complete 1,516,355-bp sequence of the type strain, the stock derived from the South African Welgevonden isolate. Only 62% of the genome is predicted to be coding sequence, encoding 888 proteins and 41 stable RNA species. The most striking feature is the large number of tandemly repeated and duplicated sequences, some of continuously variable copy number, which contributes to the low proportion of coding sequence. These repeats have mediated numerous translocation and inversion events that have resulted in the duplication and truncation of some genes and have also given rise to new genes. There are 32 predicted pseudogenes, most of which are truncated fragments of genes associated with repeats. Rather then being the result of the reductive evolution seen in other intracellular bacteria, these pseudogenes appear to be the product of ongoing sequence duplication events.
 
Date 2005-01-18
2012-07-09T05:56:37Z
2012-07-09T05:56:37Z
 
Type Journal Article
 
Identifier Collins, N.E., Liebenberg, J., Villiers, E.P. de, Brayton, K.A., Louw, E., Pretorius, E., Faber, F.E., Heerden, H. van, Josemans, A., Kleef, M. van, Steyn, H.C., Strijp, M.F. van, Zweygarth, E., Jongejan, F., Maillard, J.C., Berthier, D., Botha, M., Joubert, F., Corton, C.H., Thomson, N.R., Allsopp, M.T. and Allsopp, B.A. 2005. The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number. Proceedings of the National Academy of Sciences 102(3): 838-843.
0027-8424
1091-6490
https://hdl.handle.net/10568/21178
https://doi.org/10.1073/pnas.0406633102
 
Language en
 
Rights Limited Access
 
Publisher Proceedings of the National Academy of Sciences
 
Source Proceedings of the National Academy of Sciences of the United States of America