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Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume

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Title Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume
 
Creator Kelemu, Segenet
Cardona, C.
Segura, G.
 
Subject feeds
feed crops
animal feeding
 
Description The tropical forage legume Clitoria ternatea (L.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. It is resistant to a number of pathogens and pests. These important traits gave us reasons to look more closely at the plant. A highly basic small protein was purified from seeds of C. ternatea to homogeneity by using ultrafiltration with Centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (IEF). A single protein band was obtained on both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and IEF gels. The protein, designated ‘finotin’, has broad and potent inhibitory effect on the growth of various important fungal pathogens of plants, namely Rhizoctonia solani, Fusarium solani, Colletotrichum lindemuthianum, Lasiodiplodia theobromae, Pyricularia grisea, Bipolaris oryzae and Colletotrichum gloeosporioides. It also inhibits the common bean bacterial blight pathogen Xanthomonas axonopodis pv. phaseoli. Moreover, finotin has powerful inhibitory properties against the bean bruchids Zabrotes subfasciatus and Acanthoscelides obtectus.
 
Date 2004-12
2012-07-09T05:53:31Z
2012-07-09T05:53:31Z
 
Type Journal Article
 
Identifier Kelemu, S., Cardona, C. and Segura, G. 2004. Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume. Plant Physiology and Biochemistry 42(11): 867-873.
0981-9428
https://hdl.handle.net/10568/21176
https://doi.org/10.1016/j.plaphy.2004.10.013
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 867-873
 
Publisher Elsevier
 
Source Plant Physiology and Biochemistry