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Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

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Title Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
 
Creator Lambson, B.
Nene, Vishvanath
Obura, M.
Shah, Tushaar
Pandit, P.
Ole-MoiYoi, O.K.
Delroux, K.
Welburn, S.C.
Skilton, Robert A.
Villiers, Etienne P. de
Bishop, Richard P.
 
Subject insects
ticks
 
Description Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.
 
Date 2005-08
2012-07-09T05:47:05Z
2012-07-09T05:47:05Z
 
Type Journal Article
 
Identifier Lambson, B., Nene, V., Obura, M., Shah, T., Pandit, P., Ole-Moiyoi, O., Delroux, K., Welburn, S., Skilton, R., Villiers, E.P. de and Bishop, R. 2005. Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells. Insect Molecular Biology 14: 403-414.
0962-1075
1365-2583
https://hdl.handle.net/10568/21174
https://doi.org/10.1111/j.1365-2583.2005.00571.x
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 403-414
 
Publisher Wiley
 
Source Insect Molecular Biology