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A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters

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Title A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters
 
Creator Javaid, Shaista
 
Contributor Amin, Imran
Jander, Georg
Mukhtar, Zahid
Saeed, Nasir A
Mansoor, Shahid
 
Description The first generation transgenic crops used strong constitutive promoters for transgene expression.
However, tissue-specific expression is desirable for more precise targeting of transgenes. Moreover,
piercing/sucking insects, which are generally resistant to insecticidal Bacillus thuringiensis (Bt) proteins,
have emerged as a major pests since the introduction of transgenic crops expressing these toxins.
Phloem-specific promoters isolated from Banana bunchy top virus (BBTV) were used for the expression
of two insecticidal proteins, Hadronyche versuta (Blue Mountains funnel-web spider) neurotoxin (Hvt)
and onion leaf lectin, in tobacco (Nicotiana tabacum). Here we demonstrate that transgenic plants
expressing Hvt alone or in combination with onion leaf lectin are resistant to Phenacoccus solenopsis
(cotton mealybug), Myzus persicae (green peach aphids) and Bemisia tabaci (silver leaf whitefly).
The expression of both proteins under different phloem-specific promoters resulted in close to 100%
mortality and provided more rapid protection than Hvt alone. Our results suggest the employment
of the Hvt and onion leaf lectin transgenic constructs at the commercial level will reduce the use of
chemical pesticides for control of hemipteran insect pests.
 
Date 2016-10-06
2017-01-11T09:24:06Z
2017-01-11T09:24:06Z
 
Type Journal Article
 
Identifier https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052614/pdf/srep34706.pdf
https://mel.cgiar.org/reporting/download/hash/mZnA01Ht
Shaista Javaid, Imran Amin, Georg Jander, Zahid Mukhtar, Nasir A Saeed, Shahid Mansoor. (6/10/2016). A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. Scientific Reports, 6.
https://hdl.handle.net/20.500.11766/5489
Open access
 
Language en
 
Rights CC-BY-4.0
 
Format PDF
 
Publisher Nature Research (part of Springer Nature) (Fully open access journals)
 
Source Scientific Reports;6,(2016)