Record Details

A Genome-Wide Association Study on the Seedless Phenotype in Banana (Musa spp.) Reveals the Potential of a Selected Panel to Detect Candidate Genes in a Vegetatively Propagated Crop

MELSpace

View Archive Info
 
 
Field Value
 
Title A Genome-Wide Association Study on the Seedless Phenotype in Banana (Musa spp.) Reveals the Potential of a Selected Panel to Detect Candidate Genes in a Vegetatively Propagated Crop
 
Creator Sardos, Julie
 
Contributor Rouard, Mathieu
Hueber, Yann
Cenci, Alberto
Hyma, Katie E.
Van Den Houwe, Ines
Hribova, Eva
Courtois, Brigitte
Roux, Nicolas
 
Subject genetic
Banana
 
Description Banana (Musa sp.) is a vegetatively propagated, low fertility, potentially hybrid and polyploid
crop. These qualities make the breeding and targeted genetic improvement of this crop a
difficult and long process. The Genome-Wide Association Study (GWAS) approach is
becoming widely used in crop plants and has proven efficient to detecting candidate genes
for traits of interest, especially in cereals. GWAS has not been applied yet to a vegetatively
propagated crop. However, successful GWAS in banana would considerably help unravel
the genomic basis of traits of interest and therefore speed up this crop improvement. We
present here a dedicated panel of 105 accessions of banana, freely available upon request,
and their corresponding GBS data. A set of 5,544 highly reliable markers revealed high levels
of admixture in most accessions, except for a subset of 33 individuals from Papua. A
GWAS on the seedless phenotype was then successfully applied to the panel. By applying
the Mixed Linear Model corrected for both kinship and structure as implemented in TASSEL,
we detected 13 candidate genomic regions in which we found a number of genes
potentially linked with the seedless phenotype (i.e. parthenocarpy combined with female
sterility). An additional GWAS performed on the unstructured Papuan subset composed of
33 accessions confirmed six of these regions as candidate. Out of both sets of analyses,
one strong candidate gene for female sterility, a putative orthologous gene to Histidine
Kinase CKI1, was identified. The results presented here confirmed the feasibility and potential
of GWAS when applied to small sets of banana accessions, at least for traits underpinned
by a few loci. As phenotyping in banana is extremely space and time-consuming,
this latest finding is of particular importance in the context of banana improvement.
 
Date 2016-05-04
2017-01-20T14:58:02Z
2017-01-20T14:58:02Z
 
Type Journal Article
 
Identifier https://mel.cgiar.org/reporting/download/hash/KTfhN7Xk
Julie Sardos, Mathieu Rouard, Yann Hueber, Alberto Cenci, Katie E. Hyma, Ines Van Den Houwe, Eva Hribova, Brigitte Courtois, Nicolas Roux. (4/5/2016). A Genome-Wide Association Study on the Seedless Phenotype in Banana (Musa spp. ) Reveals the Potential of a Selected Panel to Detect Candidate Genes in a Vegetatively Propagated Crop. PLoS ONE, 5 (11).
https://hdl.handle.net/20.500.11766/5531
Open access
 
Language en
 
Rights CC-BY-4.0
 
Format PDF
 
Publisher Public Library of Science (PLOS ONE)
 
Source PLoS ONE;5,(2016)