Record Details

Characterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markers

MELSpace

View Archive Info
 
 
Field Value
 
Title Characterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markers
 
Creator Kumar, Harish
 
Contributor Sarker, Ashutosh
 
Subject genetic
 
Description Forty-one elite lentil lines were studied for stability of grain Fe and Zn concentration across three locations (New Delhi, Ludhiana
and Pantnagar) in India. The stability analysis was carried using Eberhart and Russel’s stability model. Pooled analysis of variance
over locations revealed highly significant differences between genotypes, locations and genotype × location interaction. The
maximum mean for grain Fe concentration over the locations was obtained for L 4704 (136.91 mg/kg grain), while for grain Zn
concentration was highest for VL 141 (81.542 mg/kg grain). The highest mean grain Fe and Zn was recorded at Indian Agricultural
Research Institute, New Delhi (Fe-87.30 mg/kg and Zn-68.602 mg/kg). Although both micronutrients were influenced by
environment, grain Fe showed more G × E interaction in comparison to grain Zn concentration. Six lentil genotypes exhibited
stability for grain Fe concentration and fifteen genotypes for grain Zn concentration were identified. The studied genotypes with
diverse micronutrients concentration were analysed for molecular diversity using 32 polymorphic SSR markers. These markers
amplified a total of 130 bands with PIC value ranging from 0.138 to 0.798. Based on SSR allelic diversity, genotypes were grouped
in two major clusters. The clustering pattern indicated variability in the studied lines. On the basis of genetic diversity for
micronutrient concentration, ten crosses are suggested for hybridization purpose to obtain the transgressive segregants. The combined
analysis of multi-location phenotyping and genetic diversity further suggests six potential crosses for developing micronutrient rich
varieties for the future.
 
Date 2014-07-31
2017-01-22T18:20:24Z
2017-01-22T18:20:24Z
 
Type Journal Article
 
Identifier http://www.cropj.com/kumar_8_7_2014_1005_1012.pdf
https://mel.cgiar.org/reporting/download/hash/3FLJH3aD
Harish Kumar, Ashutosh Sarker. (31/7/2014). Characterization of grain iron and zinc in lentil (Lens culinaris Medikus culinaris) and analysis of their genetic diversity using SSR markers. Australian journal of crop science, 8 (7), pp. 1005-1012.
https://hdl.handle.net/20.500.11766/5536
Open access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format PDF
 
Publisher Southern Cross Publishing Group
 
Source Australian journal of crop science;8,(2014) Pagination 1005-1012