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Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

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Title Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host
 
Creator Ali, Iftikhar
 
Contributor Akhtar, Khalid Pervaiz
Akbar, Fazal
Hassan, Ishtiaq
Amin, Imran
Saeed, Muhammad
Mansoor, Shahid
 
Subject betasatellite
clcumub
Wheat
 
Description Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected
scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the
lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between A rich sequence and βC1 gene and insertion of 27 nt in the middle of βC1 ORF. This study may help in investigating molecular basis of resistance in G. arboreum.
 
Date 2016-02-01
2017-02-09T16:29:11Z
2017-02-09T16:29:11Z
 
Type Journal Article
 
Identifier https://mel.cgiar.org/reporting/download/hash/uuW05ZrO
Iftikhar Ali, Khalid Pervaiz Akhtar, Fazal Akbar, Ishtiaq Hassan, Imran Amin, Muhammad Saeed, Shahid Mansoor. (1/2/2016). Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host. The Plant Pathology Journal, 32(1), pp. 47-52.
https://hdl.handle.net/20.500.11766/5647
Open access
 
Language en
 
Rights CC-BY-4.0
 
Format PDF
 
Publisher Hanrimwon Publishing Company
 
Source The Plant Pathology Journal;32,(2016) Pagination 47,52