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Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus.

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Title Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus.
 
Creator Mustafa, Roma
 
Contributor Shafiq, Muhammad
Mansoor, Shahid
Briddon, Rob W.
Scheffler, Brian
Scheffler, Jodi
Amin, Imran
 
Subject Wheat
 
Description The study described here has optimized the conditions for virus-induced gene silencing (VIGS) in three cultivated cotton species (Gossypium hirsutum, G. arboreum, and G. herbaceum) using a Tobacco rattle virus (TRV) vector. The system was used to silence the homolog of the Arabidopsis thaliana chloroplastos alterados 1 (AtCLA1) gene, involved in chloroplast development, in G. herbaceum, G. arboreum, and six commercial G. hirsutum cultivars. All plants inoculated with the TRV vector to silence CLA1 developed a typical albino phenotype indicative of silencing this gene. Although silencing in G. herbaceum and G. arboreum was complete, silencing efficiency differed for each G. hirsutum cultivar. Reverse transcriptase polymerase chain reaction (PCR) and real-time quantitative PCR showed a reduction in mRNA levels of the CLA1 homolog in all three species, with the highest efficiency (lowest CLA1 mRNA levels) in G. arboreum followed by G. herbaceum and G. hirsutum. The results indicate that TRV is a useful vector for VIGS in Gossypium species. However, selection of host cultivar is important. With the genome sequences of several cotton species recently becoming publicly available, this system has the potential to provide a very powerful tool for the rapid, large-scale reverse-genetic analysis of genes in Gossypium spp.
 
Date 2015-11-24
2017-02-10T00:13:05Z
2017-02-10T00:13:05Z
 
Type Journal Article
 
Identifier http://link.springer.com/article/10.1007/s12033-015-9904-z
https://mel.cgiar.org/reporting/download/hash/GSjtvVnV
Roma Mustafa, Muhammad Shafiq, Shahid Mansoor, Rob W. Briddon, Brian Scheffler, Jodi Scheffler, Imran Amin. (24/11/2015). Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp. ) Using Tobacco Rattle Virus. Molecular Biotechnology, 58(1), pp. 65-72.
https://hdl.handle.net/20.500.11766/5660
Limited access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format ZIP
 
Publisher Springer Verlag (Germany)
 
Source Molecular Biotechnology;58,(2015) Pagination 65,72