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RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly

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Title RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly
 
Creator Raza, Amir
 
Contributor Jamil, Hassan
Shafiq, Muhammad
Amin, Imran
Scheffler, Jodi
Scheffler, Brian
Mansoor, Shahid
 
Description Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) that could offer great potential for insect pest management. The diet of insects feeding
exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends on the osmotic pressure within the insect body. Based on this physiological mechanism, transgenic plants of Nicotiana tabacum were generated expressing double stranded RNA (dsRNA) against both aquaporin (AQP) and a sucrase gene, alpha glucosidase (AGLU). These two genes are involved in osmotic pressuremaintenance particularly in sap sucking insects, and the aim was to disrupt osmoregulationwithin the insect ultimately leading to mortality. Real time quantitative PCR (RTqPCR) was performed to assess the suppression of gene expression in Bemisia tabaci (B. tabaci) and mortality was recorded during transgenic tobacco feeding bioassays. Feeding of insects on plants expressing dsRNA significantly reduced the transcript level of the target genes in B. tabaci after six days of feeding and more than 70% mortality was observed in B. tabaci fed on transgenic plants compared to the control plants. Our data shows that downregulation of genes related to osmoregulation may find practical applications for the control
of this important pest in cotton and other crops.
 
Date 2016-04-19
2017-02-10T00:15:18Z
2017-02-10T00:15:18Z
 
Type Journal Article
 
Identifier http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0153883&type=printable
https://mel.cgiar.org/reporting/download/hash/lUrSy9vI
Amir Raza, Hassan Jamil, Muhammad Shafiq, Imran Amin, Jodi Scheffler, Brian Scheffler, Shahid Mansoor. (19/4/2016). RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly. PLoS One, 11 (4), pp. 1-13.
https://hdl.handle.net/20.500.11766/5661
Open access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format PDF
 
Publisher Public Library of Science (PLOS ONE)
 
Source PLoS ONE;11,(2016) Pagination 1-13