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Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species

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Title Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species
 
Creator Singh, A.K.
 
Contributor Dikshit, H K
Singh, Dharmendra
Jain, Niveta
Aski, Muraleedhar
Sarker, Ashutosh
Sharma, T. R.
 
Subject genetic
 
Description Expressed sequence tag-simple sequence repeat (EST-SSR) markers were used to analyse genetic diversity among three Lens species. The SSR loci amplified successfully in wild species, with 94·82% transferability in Lens culinaris subsp. orientalis, 95·4% in Lens nigricans, 98·81% in L. culinaris subsp. odemensis, 94·82% in L. culinaris subsp. tomentosus and 96·55% in Lens ervoides. Ninety-nine alleles (average 3·41 alleles/locus) were detected by 29 SSR markers. Based on the unweighted pair group method with arithmetic mean cluster analysis, all the genotypes were grouped into three clusters at a similarity level of 0·30. The diversity analysis indicated no species-specific clustering of the wild and cultivated species. Wild species L. nigricans and L. culinaris subsp. ode- mensis, L. culinaris subsp. orientalis and L. ervoides were grouped in Cluster I, whereas the Mediterranean land races of L. culinaris subsp. culinaris and L. culinaris subsp. tomentosus formed a separate group in Cluster II A. Cluster II B comprised L. ervoides, L. culinaris subsp. orientalis and L. culinaris subsp. culinaris. Clusters II C, II D and II F included cultivated Indian lentil genotypes. Cluster II E comprised Indian and Mediterranean germplasm lines. Cluster II F included three early maturing germplasm lines, whereas Cluster III included only two germplasm lines. The functional annotation of SSR-containing unigenes revealed that a majority of genes were involved in an important transport-related function or were a component of metabolic pathways. A high level of polymorphism of EST-SSRs and their transferability to related wild species indicated that these markers could be used for molecular screening, map construction, comparative genomic studies and marker-assisted selection.
 
Date 2016-08-28
2017-02-23T12:55:02Z
2017-02-23T12:55:02Z
 
Type Journal Article
 
Identifier https://mel.cgiar.org/dspace/limited
A. K. Singh, H K Dikshit, Dharmendra Singh, Niveta Jain, Muraleedhar Aski, Ashutosh Sarker, T. R. Sharma. (28/8/2016). Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species. Journal of Agricultural Science, 154 (7), pp. 1254-1269.
https://hdl.handle.net/20.500.11766/5896
Limited access
 
Language en
 
Format PDF
 
Publisher Cambridge University Press (CUP)
 
Source Journal of Agricultural Science;154,(2016) Pagination 1254-1269