Record Details

CIMMYT Institutional Multimedia Publications Repository

View Archive Info
 

Metadata

 
Field Value
 
Title Soluble peroxidase activity in maize endosperm associated with maize weevil resistance
 
Names Garcia-Lara, S.
Arnason, J.T.
Diaz-Pontones, D.
Gonzalez, E.
Bergvinson, D.J.
Date Issued 2007 (iso8601)
Abstract Plant peroxidases (PODs) are involved in resistance to pathogens and insects. This study investigated the role of POD in maize (Zea mays L.) resistance to the maize weevil, Sitophilus zeamais (Motsch.). Insect bioassays were performed under controlled conditions to assess maize weevil resistance. Peroxidase activity was measured in the major grain tissues using guaiacol and peroxide. Signifi cant variation (P > 0.001) among genotypes was observed in both the insect bioassay traits and POD activity. Peroxidase was detected in the embryo, endosperm, and pedicel, but it was not detected in the pericarp. Signifi cant correlations were found between endosperm POD activity and maize weevil resistance (r = 0.89, P < 0.001). Histological staining confi rmed POD activity in the vascular cylinder of the embryo, while activity in the endosperm was restricted to the aleurone layer. This study shows that POD activity is correlated with maize weevil resistance and may be used as a potential biochemical marker.
Genre Article
Access Condition Restricted Access
Identifier http://hdl.handle.net/10883/3046