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Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia

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Title Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia
 
Creator Rouatbi, Mariem
 
Contributor Amdouni, Yosra
Amairia, Safa
Rijeibi, Mohammed
Sammoudi, Said
Rekik, Mourad
Gharbi, Mohamed
 
Subject sidi bouzid
lamb
beja
 
Description Toxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan Toxoplasma gondii. It can infect all warm-blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with T. gondii in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of T. gondii. Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja. DNA was extracted from the samples using the Wizard" genomic DNA purification kit. A nested PCR using two pairs of primers (NN 1 and NN2, Tg-NP1 and Tg-NP2) were used to detect infection with T. gondii, which was then confirmed by sequencing. Eight T. gondii amplicons were sequenced (accession number KT896498) and deposited in GenBank. The T. gondii amplicons showed 97–100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested PCR detected T. gondii DNA in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of T. gondii infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.
 
Date 2017-12-20
2017-02-27T14:46:01Z
2017-02-27T14:46:01Z
 
Type Journal Article
 
Identifier http://onlinelibrary.wiley.com/doi/10.1002/vms3.53/epdf
https://www.researchgate.net/publication/307979978_Molecular_Detection_and_Phylogenetic_Analyses_of_Toxoplasma_gondii_from_Naturally_Infected_Sheep_in_Northern_and_Central_Tunisia
https://mel.cgiar.org/reporting/download/hash/UBXL90nO
Mariem Rouatbi, Yosra Amdouni, Safa Amairia, Mohammed Rijeibi, Said Sammoudi, Mourad Rekik, Mohamed Gharbi. (20/12/2017). Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia. Veterinary Medicine and Science, 3 (1), pp. 22-31.
https://hdl.handle.net/20.500.11766/6072
Open access
 
Language en
 
Rights CC-BY-4.0
 
Format PDF
 
Publisher John Wiley and Sons
 
Source Veterinary Medicine and Science;3,(2017) Pagination 22-31