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Identification of molecular markers associated with rust resistance genes in lentil (Lens culinaris sub sp. culinaris)

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Title Identification of molecular markers associated with rust resistance genes in lentil (Lens culinaris sub sp. culinaris)
 
Creator Fikru, Mekonnen
 
Contributor Are, Ashok Kumar
Agrawal, Shiv Kumar
Kemal, Seid Ahmed
Gill, Ranjit Kaur
Singh, Sarvjeet
Sharma, Tilak Raj
 
Subject pests and disease
 
Description Lentil rust caused by Uromyces vicia- fabae (Pers.) J. Schroet is the most widespread and economically
important disease in Ethiopia, Bangladesh, India, Nepal, and Morocco. The objective of this study was
to determine the genetics of rust resistance in lentil and to locate map position of gene(s) associated with
resistance to rust and to identify molecular markers that can be used in marker-assisted selection. We
report here the mapping of rust resistance gene using lentil specific SSR markers. For this purpose, a
recombinant inbred line (RILs) population comprising of 133 lines, derived from the cross of a rust
resistant line (FLIP-2004-7L) as male parent, and a rust susceptible line (L-9-12) as female parent was
used. Phenotyping of the RIL population was carried out at rust hot spots in Chefe Donsa, Sinana and
Sirinka (Ethiopia) during summers of 2011 and 2012 and at Gurdaspur, India during 2011/12 cropping
season. The mapping population segregated in 1:1 ratio (χ2= 0.008), indicating that lentil rust resistance
is controlled by a single gene. Linkage analysis for rust resistance gene showed that the SSR marker
GLLC106 was located at a distance of 10cM from resistance gene. All resistant RILs had bands at 200bp
region. This co-segregating marker can be used for marker-assisted selection in a lentil breeding program
aimed at developing lentil cultivars resistant to rust disease. However, future research should be aimed
at developing additional markers closer to the resistance gene so that the region is saturated.
 
Date 2017-03-12T22:17:13Z
2017-03-12T22:17:13Z
 
Type Poster
 
Identifier http://www.icarda.org/sites/default/files/u158/ICP2016-Conference-abstract-book.pdf 
https://mel.cgiar.org/reporting/downloadmelspace/hash/ioQgmM3H/v/bd9db3f5dd51320037816e039f752a8f
Mekonnen Fikru, Ashok Kumar Are, Shiv Kumar Agrawal, Seid Ahmed Kemal, Ranjit Kaur Gill, Sarvjeet Singh, Tilak Raj Sharma. (31/12/2016). Identification of molecular markers associated with rust resistance genes in lentil (Lens culinaris sub sp. culinaris).
https://hdl.handle.net/20.500.11766/6420
Open access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format PDF