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QTL mapping of pearl millet rust resistance using an integrated DArT- and SSR-based linkage map

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Title QTL mapping of pearl millet rust resistance using an integrated DArT- and SSR-based linkage map
 
Creator Ambawat, Supriya
 
Contributor Senapathy, Senthilvel
Hash, Charles
Nepolean, Thirunavukkarasu
Vengaldas, Rajaram
Eshwar, Kankanti
Sharma, Rajan
Thakur, Ram P.
Rao, Veeranki P.
Yadav, Ram C.
Srivastava, Rakesh
 
Subject linkage map
rust resistance
diversity arrays technology
 
Description Rust, caused by the fungus Puccinia
substriata var. indica, is one of the most important
production constraints of pearl millet worldwide,
leading to grain yield losses of up to 76 % as well as
major losses in fodder yield and quality. Here, we
report the development of a linkage map integrating
Diversity Arrays Technology (DArT) markers and
simple sequence repeat (SSR) markers, using this to
identify quantitative trait loci (QTLs) for pearl millet
rust resistance. Genotyping data from 256 DArT and
70 SSR markers on 168 F7 recombinant inbred lines
from cross 81B-P6 9 ICMP 451-P8 were used to
construct a linkage map comprised of 286 loci (229
DArT and 57 SSR markers) spanning a total length of
740.3 cM (Haldane) with an average adjacent marker
distance of 2.7 cM. Linkage group 7 (LG7)
(153.5 cM) was the longest and LG6 the shortest (45.0 cM). The map was used to identify a major QTL
for rust resistance with an LOD score of 27 on LG1,
which explained 58 % of the observed phenotypic
variation. In addition, two putative modifiers of small
effect were detected, one each on LG4 and LG7. The
novel rust resistance QTL identified on LG1 is thought
to confer a durable slow-rusting phenotype, which is
still effective in India more than 20 years after it was
first deployed in the previously popular single-cross
hybrid MH 179 (ICMH 451). The flanking markers
reported here provide a framework for marker-assisted
selection and possible future map-based cloning of
this resistance gene.
 
Date 2016-03-16
2017-03-15T04:01:40Z
2017-03-15T04:01:40Z
 
Type Journal Article
 
Identifier http://oar.icrisat.org/id/eprint/9400
https://mel.cgiar.org/reporting/download/hash/faGiAZxW
Supriya Ambawat, Senthilvel Senapathy, Charles Hash, Thirunavukkarasu Nepolean, Rajaram Vengaldas, Kankanti Eshwar, Rajan Sharma, Ram P. Thakur, Veeranki P. Rao, Ram C. Yadav, Rakesh Srivastava. (16/3/2016). QTL mapping of pearl millet rust resistance using an integrated DArT- and SSR-based linkage map. Euphytica, 209, pp. 461-476.
https://hdl.handle.net/20.500.11766/6484
Limited access
 
Language en
 
Rights CC-BY-NC-4.0
 
Format PDF
 
Publisher Springer Verlag (Germany)
 
Source Euphytica;209,(2016) Pagination 461-476